Patterns & Phenotypes
Peroxisome biogenesis occurs in late dorsal-anterior structures in the development of Xenopus laevis
Article first published online: 31 OCT 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Stem Cells
Volume 236, Issue 12, pages 3554–3561, December 2007
How to Cite
Cooper, C. A., Walsh, L. A. and Damjanovski, S. (2007), Peroxisome biogenesis occurs in late dorsal-anterior structures in the development of Xenopus laevis. Dev. Dyn., 236: 3554–3561. doi: 10.1002/dvdy.21370
- Issue published online: 14 NOV 2007
- Article first published online: 31 OCT 2007
- Manuscript Accepted: 2 OCT 2007
- Natural Sciences and Engineering Research Council (NSERC)
- Xenopus laevis;
Metabolism and development are two important processes not often examined in the same context. The focus of the present study is the expression of specific peroxisomal genes, the subsequent biogenesis of peroxisomes, and their potential role in the metabolism associated with the development of Xenopus laevis embryos. The temporal and expression patterns of six peroxisomal genes (PEX5, ACO, PEX19, PMP70, PEX16, and catalase) were elucidated using RT-PCR. Functionally related peroxisomal genes exhibited similar expression patterns with their RNA levels elevated relatively late during embryogenesis. Using immunohistochemistry PMP70 and catalase protein was localized largely to dorsal-anterior structures. Peroxisomal function was assayed with peroxisomal targeted-GFP, which when microinjected, revealed peroxisomes in dorsal-anterior structures at stage 45. A requirement for peroxisomal function appears to be present only late in development as organogenesis is finishing, yolk stores are depleted, and ingestion commences. Developmental Dynamics 236:3554–3561, 2007. © 2007 Wiley-Liss, Inc.