Drs. Chaw and Vance contributed equally to this work.
Gastrulation in the spider Zygiella x-notata involves three distinct phases of cell internalization
Article first published online: 9 NOV 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Stem Cells
Volume 236, Issue 12, pages 3484–3495, December 2007
How to Cite
Chaw, R. C., Vance, E. and Black, S. D. (2007), Gastrulation in the spider Zygiella x-notata involves three distinct phases of cell internalization. Dev. Dyn., 236: 3484–3495. doi: 10.1002/dvdy.21371
- Issue published online: 14 NOV 2007
- Article first published online: 9 NOV 2007
- Manuscript Accepted: 29 SEP 2007
- Mellon grant to Reed College
- Betty C. Liu Memorial Biology Research Fellowship
- HHMI student research grants
The cell movements of gastrulation were analyzed in embryos of the spider Zygiella x-notata, using time-lapse video, cell tracing, and improved histology. Cells are internalized near the center of the germ disc in three distinct phases. First, cumulus mesenchyme cells ingress and migrate as a group beneath the superficial layer. Second, mass internalization through a blastopore yields a diffusely organized deep layer. Third, superficial cells accumulate at the center of the germ disc to form the caudal bud. The floor is internalized, and the caudal bud moves over the nascent dorsal field to form the caudal lobe. This pattern of gastrulation differs from the canonical pattern described in the historical literature: (1) the cumulus of Z. x-notata is completely formed before any other cells internalize; and (2) the caudal lobe is formed by means of the caudal bud, which is a locus of cell internalization. Developmental Dynamics 236:3484–3495, 2007. © 2007 Wiley-Liss, Inc.