Drs. Chaw and Vance contributed equally to this work.
Gastrulation in the spider Zygiella x-notata involves three distinct phases of cell internalization
Article first published online: 9 NOV 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Stem Cells
Volume 236, Issue 12, pages 3484–3495, December 2007
How to Cite
Chaw, R. C., Vance, E. and Black, S. D. (2007), Gastrulation in the spider Zygiella x-notata involves three distinct phases of cell internalization. Dev. Dyn., 236: 3484–3495. doi: 10.1002/dvdy.21371
- Issue published online: 14 NOV 2007
- Article first published online: 9 NOV 2007
- Manuscript Accepted: 29 SEP 2007
- Mellon grant to Reed College
- Betty C. Liu Memorial Biology Research Fellowship
- HHMI student research grants
The Supplementary Material referred to in this article can be found at http://www.interscience.wiley.com/jpages/1058-8388/suppmat
|dvdy21371-DVDY213711.mov||5728K||Supplementary Movie 1.Low-resolution timelapse from blastoderm (Stage C) through hatching (Stage K). Posterior down. Elapsed time approx. 271 hr.|
|dvdy21371-DVDY213712.mov||1684K||Supplementary Movie 2.Timelapse of cumulus formation and migration. Cumulus mesenchyme cells are false-colored red; some cells that will ingress later, during gastrulation II, are false-colored in different colors. Cells were traced frame-by-frame, but movie shows one-hr intervals only. Posterior down. Elapsed time approx. 20 hr.|
|dvdy21371-DVDY213713.mov||410K||Supplementary Movie 3.Timelapse of gastrulation II. Some ingressing cells are false-colored. Movie starts at cumulus formation (cumulus comprises the unlabeled, apically constricted cells at center) and runs through gastrulation II. Cells were traced frame-by-frame, but movie shows one-hr intervals only. Posterior down. Elapsed time approx. 24 hr.|
|dvdy21371-DVDY213714.mov||2514K||Supplementary Movie 4.Timelapse of gastrulation II showing movement of surface cells toward and internalization at a central blastopore. Blastopore forms slightly above and to the right of the starting position of the cumulus. Cumulus migrates toward lower left. Elapsed time approx. 15 hr.|
|dvdy21371-DVDY213715.mov||3762K||Supplementary Movie 5.Timelapse of gastrulation I, II, and III (Stages CH). Centrifugal movement of cells internalized during gastrulation II is particularly apparent, as is gastrulation III. Cumulus migrates toward lower left. Elapsed time approx. 45 hr.|
|dvdy21371-DVDY213716.mov||4977K||Supplementary Movie 6.Timelapse spanning cumulus migration through caudal bud movement (Stages DI). Lateral view, posterior to left. Movement of caudal bud is particularly apparent. Elapsed time approx. 40 hr. Movie courtesy of David Rasmussen.|
|dvdy21371-DVDY213717.mov||361K||Supplementary Movie 7.Timelapse of dorsal field formation. Some dorsal field cells are false-colored. Cells were traced frame-by-frame, but movie shows five-hr intervals only. Posterior down. Elapsed time approx. 40 hr.|
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