Multicellular rosette formation during cell ingression in the avian primitive streak
Article first published online: 10 DEC 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 237, Issue 1, pages 91–96, January 2008
How to Cite
Wagstaff, L. J., Bellett, G., Mogensen, M. M. and Münsterberg, A. (2008), Multicellular rosette formation during cell ingression in the avian primitive streak. Dev. Dyn., 237: 91–96. doi: 10.1002/dvdy.21390
- Issue published online: 19 DEC 2007
- Article first published online: 10 DEC 2007
- Manuscript Accepted: 23 OCT 2007
- British Heart Foundation. Grant Number: PG03/041/15277
- Wellcome Trust. Grant Number: JIF 063722
- primitive streak;
Cell movements are a fundamental feature during the development of multi-cellular organisms. In amniote gastrulation, cells ingress through the primitive streak, which identifies the anterior-posterior axis of the embryo. We investigated the cytoskeletal architecture during these morphogenetic processes and characterized microtubule organisation in whole chick embryos. This revealed the distribution of cells with polarized and radial microtubule (MT) arrays across different regions of the embryo. Cells in the epiblast usually displayed radial MT-arrays, while the majority of cells in the primitive streak had polarized MT-arrays. Within the primitive streak, many cells organized into groups and were arranged in rosette-like structures with a distinct centre characterized by an accumulation of actin. Extended confocal microscopy and three-dimensional image reconstruction identified tips of polarized cells that were protruding from the plane of rosettes, usually from the centre. We propose that organization into higher order structures facilitates cell ingression during gastrulation. Developmental Dynamics 237:91–96, 2008. © 2007 Wiley-Liss, Inc.