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dvdy21413-DVDY21413FigureS1.eps2773KFigure S1. Immunofluorescence staining for T1α showing alveolar type I cells. T1α was detected by hamster polyclonal antibody against mouse T1α. Representative photomicrograph of immunofluorescence for T1α in wild-type adult lung showing positive staining in alveolar type I cell membranes (green). [20× magnification; blue DAPI counterstain showing nuclear staining; Note: Despite clear staining, it is difficult to associate positively-stained alveolar membranes to individual alveolar type I cells]
dvdy21413-DVDY21413FigureS2.eps2765KFigure S2. Immunohistochemistry for BrdU labeling. BrdU was injected into pregnant dams at E18 stage 2 h prior to sacrifice and fetal tissue was harvested and assayed for BrdU labeling. (A) Representative photomicrograph of BrdU labeling in wild-type and transgenic lungs [10× magnification; n = 3 animals per group]. (B) Morphometric quantification of BrdU-positive cells. [Data represent cumulative number of cells positive per high power field; 4 measurements from n = 3 lungs per group. Data are expressed as Mean ± SEM; *p < 0.05]
dvdy21413-DVDY21413FigureS3.eps2763KFigure S3. Immunohistochemistry for NF-kB in FO embryo #8. NF-kB was detected in epithelial distribution by rabbit polyclonal antibody generated against RelA in FO embryo #8. [20× magnification]
dvdy21413-DVDY21413FigureS4.eps2766KFigure S4. Comparison of the mouse and human RelA sequences. (A) Nucleotide sequence comparison between mouse RelA transgenic construct and human RelA GenBank sequence (NM_021975) using NCBI BLAST2 website: (B) Protein sequence comparison between mouse RelA GenPept sequence (NP_033071) and human RelA GenPept sequence (NP_068810) using SIM Alignment Tool website: [Red letters indicate individual differences in nucleotide or protein sequence between mouse vs. human]

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