System for tamoxifen-inducible expression of cre-recombinase from the Foxa2 locus in mice

Authors

  • Eon Joo Park,

    1. Departments of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah
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  • Xiaoxia Sun,

    1. Institute of Biosciences and Technology, Texas A&M System Health Science Center, Texas A&M University, College Station, Texas
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  • Peter Nichol,

    1. Department of Surgery, University of Utah, Salt Lake City, Utah
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  • Yukio Saijoh,

    1. Departments of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah
    2. Program in Human Molecular Biology and Genetics, University of Utah, Salt Lake City, Utah
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  • James F. Martin,

    1. Institute of Biosciences and Technology, Texas A&M System Health Science Center, Texas A&M University, College Station, Texas
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  • Anne M. Moon

    Corresponding author
    1. Departments of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah
    2. Program in Human Molecular Biology and Genetics, University of Utah, Salt Lake City, Utah
    3. Department of Pediatrics, University of Utah, Salt Lake City, Utah
    • Eccles Institute of Human Genetics, Room 4160, 15 North 2030 East, Salt Lake City, UT 84112
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Abstract

To study the roles of key transcription factor networks, growth factors, and signaling molecules in the endoderm, notochord, and floorplate, we developed an inducible Cre-expressing system for altering gene function in this tissue. We generated an allele of Foxa2 that directs a tamoxifen-regulated Cre in the Foxa2 expression domain (Foxa2mcm). Activity of Foxa2mcm recapitulates endogenous Foxa2 expression in endoderm, notochord, and floorplate. Efficiency of the system in a given tissue type was dose- and timing-dependent. By comparing efficiency and location of Cre activity after administration of tamoxifen by oral gavage vs. intraperitoneal injection, we found that oral gavage achieves more rapid, robust recombination with less embryonic toxicity. This system will be useful for controlling the activity of floxed alleles at multiple stages of mouse embryogenesis and fetal development. Developmental Dynamics 237:447–453, 2008. © 2007 Wiley-Liss, Inc.

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