Patterns & Phenotypes
Gene expression analysis on sections of zebrafish regenerating fins reveals limitations in the whole-mount in situ hybridization method
Article first published online: 31 DEC 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 237, Issue 2, pages 417–425, February 2008
How to Cite
Smith, A., Zhang, J., Guay, D., Quint, E., Johnson, A. and Akimenko, M.A. (2008), Gene expression analysis on sections of zebrafish regenerating fins reveals limitations in the whole-mount in situ hybridization method. Dev. Dyn., 237: 417–425. doi: 10.1002/dvdy.21417
- Issue published online: 21 JAN 2008
- Article first published online: 31 DEC 2007
- Manuscript Accepted: 16 OCT 2007
- Canadian Institutes of Health Research (CIHR). Grant Number: MOP57896
- Ontario Government Scholarship in Science and Technology (OGSST)
- in situ hybridization;
- fin regeneration;
The caudal fin of adult zebrafish is used to study the molecular mechanisms that govern regeneration processes. Most reports of gene expression in regenerating caudal fins rely on in situ hybridization (ISH) on whole-mount samples followed by sectioning of the samples. In such reports, expression is mostly confined to cells other than those located between the dense collagenous structures that are the actinotrichia and lepidotrichia. Here, we re-examined the expression of genes by performing ISH directly on cryo-sections of regenerates. We detected expression of some of these genes in cell types that appeared to be non-expressing when ISH was performed on whole-mount samples. These results demonstrate that ISH reagents have a limited capacity to penetrate between the regenerating skeletal matrices and suggest that ISH performed directly on fin sections is a preferable method to study gene expression in fin regenerates. Developmental Dynamics 237:417–425, 2008. © 2007 Wiley-Liss, Inc.