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dvdy21418-DVDY21418SuppFig1.eps9999KSuppl. Fig. 1.A:The evolutionary relationship ofE. coliAlkB, the human homologue ALKBH1, and other human AlkB homologues. ALKBH1 is 52% similar and 23% identical to theE. coliAlkB. AlkB, ALKBH1, as well as ABH2 and ABH3, the best-characterized mammalian AlkB family members, are in bold.B:CLUSTALW Alignment of ALKBH1 homologues in highly divergent species. *The start of the deleted Alkbh1 exon 3, resulting in a frame-shifted protein.
dvdy21418-DVDY21418SuppFig2.eps21480KSuppl. Fig. 2.A:Four-week-oldWtandAlkbh1 −/− mice.B:Birth weights ofAlkbh1 +/+ , Alkbh1 +/− , andAlkbh1 −/− mice are shown for four litters.C:4311/ Tpbpin situ hybridization of e15.5Alkbh1 −/− andWtplacentas. Blue color indicatesTpbpanti-sense probe hybridization. H+E stained adjacent sections are also shown.D:Areas of maternal and fetal vessels in the placenta ofAlkbh1 −/− and wild-type embryos at E12.5. Placental sections were subjected to hematoxylin-eosin staining for detection of maternal vessels (containing denucleated red blood cells) and fetal vessels (containing nucleated red blood cells). The areas of maternal and fetal vessels were measured by image analysis (NIH image Software). Twelve different fields (magnification, 60×) for each genotype were randomly selected for analysis, and the means ± standard errors of the means of the percentage area of each vessel type in each field were calculated. n.s., not significant atP= 0.05,t -test).

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