Somite cell cycle analysis using somite-staging to measure intrinsic developmental time
Article first published online: 21 JAN 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 2, pages 377–392, February 2008
How to Cite
Venters, S. J., Hultner, M. L. and Ordahl, C. P. (2008), Somite cell cycle analysis using somite-staging to measure intrinsic developmental time. Dev. Dyn., 237: 377–392. doi: 10.1002/dvdy.21424
- Issue published online: 21 JAN 2008
- Article first published online: 21 JAN 2008
- Manuscript Accepted: 28 NOV 2007
- cell division;
- segmentation clock;
Somite stages were employed as units of intrinsic developmental time to measure cell doubling rate and other cell cycle parameters of chick forelimb level somites. Somite cell nuclei doubled over an interval corresponding to approximately 7+ somite stages (7+ ss; ∼11 hr) and approximately 24 new primary myotome cells are born per somite stage (∼16/hr). FACS analysis of DNA content in dissociated paraxial mesoderm cells indicated that slightly more than half are in G1/G0 phase of the cell cycle and that the average combined length of the S phase and G2 phase intervals is approximately 3 ss (∼4.5 hr). A wavefront of increased mitotic nuclei per segment coincident with somite budding potentially reflects a surge in the number of cells entering S phase 3 ss earlier as each PSM segment becomes unresponsive to FGF signaling as it passes through the determination front. Developmental Dynamics 237:377–392, 2008. © 2008 Wiley-Liss, Inc.