Patterns & Phenotypes
Systematic localization of oct-3/4 to the gastrulating mouse conceptus suggests manifold roles in mammalian development
Article first published online: 21 JAN 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 2, pages 464–475, February 2008
How to Cite
Downs, K. M. (2008), Systematic localization of oct-3/4 to the gastrulating mouse conceptus suggests manifold roles in mammalian development. Dev. Dyn., 237: 464–475. doi: 10.1002/dvdy.21438
- Issue published online: 21 JAN 2008
- Article first published online: 21 JAN 2008
- Manuscript Accepted: 23 NOV 2007
- National Institutes of Health. Grant Number: RO1HD042706
- primordial germ cells;
- stem cells;
- umbilical cord;
Oct-3/4 was localized to the mouse conceptus between the onset of gastrulation and 16-somite pairs (-s; ∼6.5–9.25 days postcoitum, dpc). Results revealed Oct-3/4 in a continuum of morphologically distinct epiblast-derived embryonic and extraembryonic tissues. In the allantois, distal-to-proximal diminution in the Oct-3/4 domain over time and co-localization with Flk-1 in angioblasts accorded with a role in vascular differentiation and the presence of a stem cell reservoir. In addition, visceral endoderm exhibited a dynamic salt-and-pepper distribution, which, combined with previous results of fate mapping and gene expression, suggested that Oct-3/4 is involved in the genesis of definitive endoderm. By 8-s, Oct-3/4 was globally down regulated in all but putative primordial germ cells (PGCs) and some allantoic cell clusters. Taken together, Oct-3/4's expression profile suggests unexpected and potentially far more versatile roles in development than have been previously appreciated. Developmental Dynamics 237:464–475, 2008. © 2008 Wiley-Liss, Inc.