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Systematic localization of oct-3/4 to the gastrulating mouse conceptus suggests manifold roles in mammalian development

  1. Karen M. Downs*

Article first published online: 21 JAN 2008

DOI: 10.1002/dvdy.21438

Issue

Developmental Dynamics

Developmental Dynamics

Volume 237, Issue 2, pages 464–475, February 2008

Additional Information

How to Cite

Downs, K. M. (2008), Systematic localization of oct-3/4 to the gastrulating mouse conceptus suggests manifold roles in mammalian development. Dev. Dyn., 237: 464–475. doi: 10.1002/dvdy.21438

Author Information

  1. Department of Anatomy, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin

*Department of Anatomy, University of Wisconsin-Madison School of Medicine and Public Health, 1300 University Avenue, Madison, WI 53706

Publication History

  1. Issue published online: 21 JAN 2008
  2. Article first published online: 21 JAN 2008
  3. Manuscript Accepted: 23 NOV 2007

Funded by

  • National Institutes of Health. Grant Number: RO1HD042706

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Keywords:

  • allantois;
  • endoderm;
  • Flk-1;
  • gastrulation;
  • heart;
  • hindgut;
  • mouse;
  • node;
  • Oct-3/4;
  • primordial germ cells;
  • PGCs;
  • pluripotency;
  • Pou5f1;
  • stem cells;
  • umbilical cord;
  • vasculogenesis

Abstract

Oct-3/4 was localized to the mouse conceptus between the onset of gastrulation and 16-somite pairs (-s; ∼6.5–9.25 days postcoitum, dpc). Results revealed Oct-3/4 in a continuum of morphologically distinct epiblast-derived embryonic and extraembryonic tissues. In the allantois, distal-to-proximal diminution in the Oct-3/4 domain over time and co-localization with Flk-1 in angioblasts accorded with a role in vascular differentiation and the presence of a stem cell reservoir. In addition, visceral endoderm exhibited a dynamic salt-and-pepper distribution, which, combined with previous results of fate mapping and gene expression, suggested that Oct-3/4 is involved in the genesis of definitive endoderm. By 8-s, Oct-3/4 was globally down regulated in all but putative primordial germ cells (PGCs) and some allantoic cell clusters. Taken together, Oct-3/4's expression profile suggests unexpected and potentially far more versatile roles in development than have been previously appreciated. Developmental Dynamics 237:464–475, 2008. © 2008 Wiley-Liss, Inc.

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