Drs. Shan, Subramaniam, and Emanuel contributed equally to this paper.
Patterns & Phenotypes
Centrifugal migration of mesenchymal cells in embryonic lung
Article first published online: 24 FEB 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 3, pages 750–757, March 2008
How to Cite
Shan, L., Subramaniam, M., Emanuel, R. L., Degan, S., Johnston, P., Tefft, D., Warburton, D. and Sunday, M. E. (2008), Centrifugal migration of mesenchymal cells in embryonic lung. Dev. Dyn., 237: 750–757. doi: 10.1002/dvdy.21462
- Issue published online: 24 FEB 2008
- Article first published online: 24 FEB 2008
- Manuscript Accepted: 17 OCT 2007
- NIH. Grant Number: 2RO1 HL44984
- alkaline phosphatase;
- smooth muscle;
- clonal analysis
Murine lung development begins at embryonic day (E) 9.5. Normal lung structure and function depend on the patterns of localization of differentiated cells. Pulmonary mesenchymal cell lineages have been relatively unexplored. Importantly, there has been no prior evidence of clonality of any lung cells. Herein we use a definitive genetic approach to demonstrate a common origin for proximal and distal pulmonary mesenchymal cells. A retroviral library with 3,400 unique inserts was microinjected into the airway lumen of E11.5 lung buds. After 7–11 days of culture, buds were stained for placental alkaline phosphatase (PLAP). Most PLAP+ cells are peribronchial smooth muscle cells, initially localized laterally near the hilum, then migrating down airways to the subpleural region. Laser-capture microdissection and polymerase chain reaction confirm the clonal identities of PLAP+ cells proximally and distally. Our observation of this fundamental process during lung development opens new avenues for investigation of maladaptive mesenchymal responses in lung diseases. Developmental Dynamics 237:750–757, 2008. © 2008 Wiley-Liss, Inc.