Retinoic acid induces prostatic bud formation
Article first published online: 4 APR 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 5, pages 1321–1333, May 2008
How to Cite
Vezina, C. M., Allgeier, S. H., Fritz, W. A., Moore, R. W., Strerath, M., Bushman, W. and Peterson, R. E. (2008), Retinoic acid induces prostatic bud formation. Dev. Dyn., 237: 1321–1333. doi: 10.1002/dvdy.21526
- Issue published online: 21 APR 2008
- Article first published online: 4 APR 2008
- Manuscript Accepted: 22 FEB 2008
- NIH. Grant Numbers: F32ES014284, F31HD049323, R37 ES01332, DK065303
- urogenital sinus;
- retinoic acid;
- bud formation;
Formation of prostatic buds from the urogenital sinus (UGS) to initiate prostate development requires localized action of several morphogenetic factors. This report reveals all-trans-retinoic acid (RA) to be a powerful inducer of mouse prostatic budding that is associated with reciprocal changes in expression of two regulators of budding: sonic hedgehog (Shh) and bone morphogenetic protein 4 (Bmp4). Localization of retinoid signaling and expression of RA synthesis, metabolism, and receptor genes in the UGS on embryonic days 14.5–17.5 implicate RA in the mechanism of bud initiation. In UGS organ culture, RA increased prostatic budding, increased Shh expression, and decreased Bmp4. Prostatic budding was stimulated in the absence of RA by recombinant SHH, by blocking BMP4 signaling with NOGGIN, or by combined treatment with SHH and NOGGIN in UGS organ culture media. These observations suggest that reciprocal changes in hedgehog and BMP signaling by RA may regulate bud initiation. Developmental Dynamics 237:1321-1333, 2008. © 2008 Wiley-Liss, Inc.