Influence of Hormones and Hormone Metabolites on the Growth of Schwann Cells Derived From Embryonic Stem Cells and on Tumor Cell Lines Expressing Variable Levels of Neurofibromin

Authors

  • Therese M. Roth,

    1. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan
    Search for more papers by this author
  • Poornapriya Ramamurthy,

    1. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan
    2. Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan
    Search for more papers by this author
  • David Muir,

    1. Department of Pediatrics, Neurology Division, University of Florida College of Medicine, Gainesville, Florida
    Search for more papers by this author
  • Margaret R. Wallace,

    1. Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida
    Search for more papers by this author
  • Yuan Zhu,

    1. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan
    2. Department of Internal Medicine-Molecular Medicine and Genetics, University of Michigan Medical School, Ann Arbor, Michigan
    Search for more papers by this author
  • Lou Chang,

    1. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan
    Search for more papers by this author
  • Kate F. Barald

    Corresponding author
    1. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan
    2. Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan
    • Department of Cell and Developmental Biology, University of Michigan Medical School, 3053 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200
    Search for more papers by this author

Abstract

A benign human plexiform neurofibroma cell. Note the extensive tubulin networks (red), which become disrupted after addition of 2ME2, an estrogen metabolite implicated in tumor toxicity. The green staining represents S100 staining, indicating the Schwann Cell origin of the tumor cells. Blue is DAPI nuclear staining. See Roth et al., Developmental Dynamics 237:513–524, 2008. © 2008 Wiley-Liss, Inc.

Ancillary