The cholinergic gene locus in amphioxus: Molecular characterization and developmental expression patterns
Article first published online: 11 APR 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 5, pages 1399–1411, May 2008
How to Cite
Candiani, S., Lacalli, T. C., Parodi, M., Oliveri, D. and Pestarino, M. (2008), The cholinergic gene locus in amphioxus: Molecular characterization and developmental expression patterns. Dev. Dyn., 237: 1399–1411. doi: 10.1002/dvdy.21541
- Issue published online: 21 APR 2008
- Article first published online: 11 APR 2008
- Manuscript Accepted: 11 MAR 2008
- MIUR. Grant Number: PRIN2006058952
- cholinergic motoneurons and interneurons;
- cholinergic gene locus;
The cholinergic gene locus (CGL), consisting of the vesicular acetylcholine transporter (VAChT)/choline acetyltransferase (ChAT) gene, encodes two specific cholinergic neuronal markers used extensively to study cholinergic transmission. In the present work, we isolated the amphioxus homologs of VAChT and ChAT and examined their expression during development. Analysis of the 5′ untranslated region of VAChT and ChAT suggests that the splicing of the VAChT/ChAT mRNA has been evolutionarily conserved in amphioxus and mammals. By double whole-mount in situ hybridization, we demonstrate that VAChT and ChAT are coexpressed in the same cells. They are first expressed in four pairs of differentiating cells in the neural plate. Their later expression is primarily in the anterior nerve cord in several types of motoneurons, some of the interneurons and in the receptor cells of the larval ocellus. Developmental Dynamics 237:1399-1411, 2008. © 2008 Wiley-Liss, Inc.