Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo
Version of Record online: 3 JUN 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 7, pages 1789–1798, July 2008
How to Cite
Mendelsohn, B. A. and Gitlin, J. D. (2008), Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Dev. Dyn., 237: 1789–1798. doi: 10.1002/dvdy.21584
- Issue online: 20 JUN 2008
- Version of Record online: 3 JUN 2008
- Manuscript Accepted: 20 APR 2008
- NIH/NIDDK. Grant Numbers: R01 44464, DK61763
- Washington University. Grant Number: T32 GM07200
- midblastula transition;
To define the mechanisms that coordinate early embryonic development and metabolism, we have examined the response of zebrafish embryos to anoxia before the midblastula transition. Our findings reveal that anoxic pre-midblastula transition embryos slow the cell cycle, arrest before the midblastula transition and can recover normally if restored to a normoxic environment. Analyses of respiratory rates reveal that pre-midblastula transition embryos are less reliant on oxidative phosphorylation than older embryos. Interestingly, arrest in anoxia occurs despite inhibition of zygotic transcription, revealing a central role for maternal factors in the response to energy limitation. Consistent with this concept, we demonstrate that the posttranslational energy-sensing AMP-activated protein kinase pathway is activated in anoxia in pre-midblastula transition embryos. Taken together, these findings demonstrate a maternal program capable of coordinating developmental rate and metabolism in the absence of transcription-based pathways or cell cycle checkpoints. Developmental Dynamics 237:1789–1798, 2008. © 2008 Wiley-Liss, Inc.