SEARCH

SEARCH BY CITATION

The Supplementary Material referred to in this article can be found at http://www.interscience.wiley.com/jpages/1058-8388/suppmat

FilenameFormatSizeDescription
sm_file_DVDY21600_1.tif6272K Figure S1. Culture between agarose sheets allows fibrillar fibronectin assembly.Fibronectin adsorbs on the surface of glass but does not form free-fibrils. A maximum projection of a z-series of confocal optical sections shows that animal cap explants cultured on glass deposit fibronectin onto the glass directly. In contrast, animal cap explants cultured between agarose pads assemble elaborate arrays of fibrillar fibronectin on the surface of the explant. A 3D projection at the XZ plane of these z-series show that fibrils do not form between cells away from either the glass substrate or the cell-agarose interface. [dvdy21600-figureS1explantsonglassandagarosePASSED.tif renamed to sm_file_DVDY21600_1.tif]
dvdy21600-figureS2protrusionPASSED.tif9114K Figure S2. Additional movements of fibrils by lamellipodia.A) Frames from another representative time-lapse of the ventral surface of the ectoderm showing lamellipodia (green; GAP43-GFP) moving a fibril (red; Cy3-coupled mAb; arrow). B) Schematic showing the cell outline (solid black line), extent of lamellipodia (dotted black line), and the fibril (red line) shown in (A) as it is drawn toward the cell (asterisk).
dvdy21600-S01A_Fig1B.mov2931K S1A_Fig1B.mov.Fibronectin fibril assembles and remodels as cells actively extend lamellipodia within a marginal zone explant. Dual wavelength confocal time-lapse sequence captured at 15 second intervals shows fibronectin fibrils (red) and scattered plasma-membrane tagged GFP mesodermal cells (green).
dvdy21600-S01B_animal_cap.mov13466K S1B_animal_cap.mov.Fibronectin fibril assembles and remodels as cells actively extend lamellipodia within an animal cap ectoderm explant. Dual wavelength confocal time-lapse sequence captured at 15 second intervals shows fibronectin fibrils (red) and scattered plasma-membrane tagged GFP mesodermal cells (green).
dvdy21600-S02_Fig2A.mov1830K S2_Fig2A.mov. Fibril Growth.High resolution confocal time-lapse sequence captured at 15 second intervals showing fibril polymerization on mesodermal cells.
dvdy21600-S03_Fig2B.mov3925K S3_Fig2B.mov. Fibril Shortening and Thickening.High resolution confocal time-lapse sequence captured at 15 second intervals showing fibrils shorten and thicken after release on ectodermal cells.
dvdy21600-S04_Fig2C.mov2589K S4_Fig2C.mov. Fibril Annealing. High resolution confocal time-lapse sequence captured at 15 second intervals showing two distinct fibrils annealing on ectodermal cells.
dvdy21600-S05_Fig2D.mov1625K S5_Fig2D.mov. Fibril Stretch/Break/Recoil.High resolution confocal time-lapse sequence captured at 15 second intervals showing a two fibrils contacting and adhering to one another on ectodermal cells.
dvdy21600-S07_Fig3D.mov1318KSupporting Information file dvdy21600-S07_Fig3D.mov
dvdy21600-S08_Fig4A.mov2646K S8_Fig4A.mov. Network collapses to cables after loss of F-actin.High resolution confocal time-lapse sequence captured at 15 second intervals after addition of 4 μM cytochalasin D to the media. The fine-network of the fibronectin fibrils collapse to thick cables that remain on the surface of the animal cap explant.
dvdy21600-S09_Fig4B.mov3147K S9_Fig4B.mov. Network collapses after Ca ++is reduced.The fibrillar fibronectin network collapses to cables and are lost from the surface of an animal cap explant after 1 mM EDTA is added to the media.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.