1097-0177/asset/DVDY_left.gif?v=1&s=b87335326ab8ecd1f573539da0b5fa6abef26532)
Special Issue Research Article
Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines
Article first published online: 15 JUL 2008
DOI: 10.1002/dvdy.21610
Copyright © 2008 Wiley-Liss, Inc.
Issue
Developmental Dynamics
Special Issue: Special Focus on the Primary Cilium
Volume 237, Issue 8, pages 2039–2052, August 2008
Additional Information
How to Cite
Nielsen, S. K., Møllgård, K., Clement, C. A., Veland, I. R., Awan, A., Yoder, B. K., Novak, I. and Christensen, S. T. (2008), Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines. Dev. Dyn., 237: 2039–2052. doi: 10.1002/dvdy.21610
Publication History
- Issue published online: 23 JUL 2008
- Article first published online: 15 JUL 2008
- Manuscript Accepted: 9 MAY 2008
Funded by
- Lundbeck Foundation and the Danish Science Research Council
- Novo Nordisk/Novozymes Foundation
- Danish Science Research Council
- Department of Biology, University of Copenhagen
- National Institutes of Health. Grant Number: RO1 R01-HD056030
- Abstract
- Article
- References
- Cited By
Keywords:
- primary cilia;
- pancreas;
- exocrine duct;
- development;
- cancer;
- Hedgehog signaling;
- Smoothened;
- Patched;
- Gli2;
- Gli3
Abstract
Hedgehog (Hh) signaling controls pancreatic development and homeostasis; aberrant Hh signaling is associated with several pancreatic diseases. Here we investigated the link between Hh signaling and primary cilia in the human developing pancreatic ducts and in cultures of human pancreatic duct adenocarcinoma cell lines, PANC-1 and CFPAC-1. We show that the onset of Hh signaling from human embryogenesis to fetal development is associated with accumulation of Hh signaling components Smo and Gli2 in duct primary cilia and a reduction of Gli3 in the duct epithelium. Smo, Ptc, and Gli2 localized to primary cilia of PANC-1 and CFPAC-1 cells, which may maintain high levels of nonstimulated Hh pathway activity. These findings indicate that primary cilia are involved in pancreatic development and postnatal tissue homeostasis. Developmental Dynamics 237:2039–2052, 2008. © 2008 Wiley-Liss, Inc.