Myotonic dystrophy protein kinase is expressed in embryonic myocytes and is required for myotube formation
Article first published online: 26 AUG 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 9, pages 2353–2366, September 2008
How to Cite
Harmon, E. B., Harmon, M. L., Larsen, T. D., Paulson, A. F. and Perryman, M. B. (2008), Myotonic dystrophy protein kinase is expressed in embryonic myocytes and is required for myotube formation. Dev. Dyn., 237: 2353–2366. doi: 10.1002/dvdy.21653
- Issue published online: 26 AUG 2008
- Article first published online: 26 AUG 2008
- Manuscript Accepted: 2 JUN 2008
- NIH. Grant Numbers: 5R01HL064136, 5P20RR017662
- South Dakota 2010 Initiative Research Center Program. Grant Number: SDBOR2010-0205
- myotonic dystrophy protein kinase;
- cardiac myocytes
Myotonic dystrophy (DM1) is a multi-systemic disease caused by a triplet nucleotide repeat expansion in the 3′ untranslated region of the gene coding for myotonic dystrophy protein kinase (DMPK). The primary pathophysiology of DM1 is thought to result from RNA transport and processing defects. The function of DMPK in development or any potential role in DM1 remains unknown. Here we report a novel role for DMPK in myogenesis. We have discovered a specific expression pattern of DMPK in mouse and chick embryonic development. DMPK is expressed in postmitotic cardiac and skeletal myocytes and developmental signaling centers. During cardiac myocyte maturation, DMPK migrates from perinuclear to cellular membrane localization. Manipulating DMPK levels in cultured cardiac and skeletal myocytes has revealed a key role for DMPK in myocyte differentiation. Overexpression of DMPK induces cell rounding and apoptosis in myocytes. In addition, DMPK is necessary for myogenin expression in differentiating C2C12 myoblasts. Developmental Dynamics 237:2353–2366, 2008. © 2008 Wiley-Liss, Inc.