Determination of microRNAs in mouse preimplantation embryos by microarray
Article first published online: 26 AUG 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 9, pages 2315–2327, September 2008
How to Cite
Yang, Y., Bai, W., Zhang, L., Yin, G., Wang, X., Wang, J., Zhao, H., Han, Y. and Yao, Y.-Q. (2008), Determination of microRNAs in mouse preimplantation embryos by microarray. Dev. Dyn., 237: 2315–2327. doi: 10.1002/dvdy.21666
- Issue published online: 26 AUG 2008
- Article first published online: 26 AUG 2008
- Manuscript Accepted: 19 MAY 2008
- National Basic Research Program of China. Grant Number: 2007CB948102
- preimplantation embryos;
Expression profile of microRNA (miRNA) in mouse oocytes and preimplantation embryos has been revealed by a novel high throughput microarray assay. A total of 97 (43 “new” and 54 known) including mouse, human, and predicted miRNAs have been discovered in the preimplantation mouse embryos which can be classified into developmental stage-dependent groups and non–stage-dependent group according to the statistical analysis of the expression patterns. Potential gene targets of each group of miRNAs are estimated by TargetsScan system and significantly changed signaling pathways and biological processes underlying these gene targets are searched by PANTHER classification system between the stage-dependent miRNAs and the non–stage-dependent miRNAs. Expression of some miRNAs is confirmed by reverse transcriptase-polymerase chain reaction. It is shown that dynamic synthesis and degradation of miRNAs coexists in the preimplantation development of mouse embryos. However, the overall quantity of miRNAs and percentage of the stage-dependent miRNAs increase as the preimplantation embryos develop. Developmental Dynamics 237:2315–2327, 2008. © 2008 Wiley-Liss, Inc.