Rescue of renal hypoplasia and cystic dysplasia in Bcl-2 −/− mice expressing Bcl-2 in ureteric bud derived epithelia
Article first published online: 26 AUG 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 9, pages 2450–2459, September 2008
How to Cite
Kondo, S., Oakes, M. G. and Sorenson, C. M. (2008), Rescue of renal hypoplasia and cystic dysplasia in Bcl-2 −/− mice expressing Bcl-2 in ureteric bud derived epithelia. Dev. Dyn., 237: 2450–2459. doi: 10.1002/dvdy.21678
- Issue published online: 26 AUG 2008
- Article first published online: 26 AUG 2008
- Manuscript Accepted: 26 JUN 2008
- National Institutes of Health. Grant Number: DK067120
- nephron numbers
Bcl-2 is the founding member of a family of proteins that influence apoptosis. Loss of bcl-2 results in renal hypoplasia/cystic dysplasia at birth. Here, we examined whether re-expression of bcl-2 throughout the ureteric bud and its derived epithelia would restore a normal renal phenotype in bcl-2 −/− mice. Re-expression of bcl-2 in the ureteric bud/collecting duct of bcl-2 −/− mice increased nephron numbers, diminished glomerular hypertrophy, and increased nephrogenic zone size. Unlike bcl-2 −/− mice which have gross renal cyst formation, few renal cysts were present in mice re-expressing bcl-2. We have previously shown increased apoptosis and proliferation, as well as aberrant protein tyrosine phosphatase 1B expression, accompanied cystic changes in bcl-2 −/− mice. These changes were not observed when bcl-2 was re-expressed in the ureteric bud/collecting duct system. Thus, expression of bcl-2 in the ureteric bud/collecting duct resulted in increased nephron numbers partially rescuing renal hypoplasia/cystic dysplasia in bcl-2 −/− mice. Developmental Dynamics 237:2450–2459, 2008. © 2008 Wiley-Liss, Inc.