Drs. Masuho and Mototani contributed equally to this work.
Dynamic expression patterns of G protein-regulated inducer of neurite outgrowth 1 (GRIN1) and its colocalization with Gαo implicate significant roles of Gαo-GRIN1 signaling in nervous system
Version of Record online: 26 AUG 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 237, Issue 9, pages 2415–2429, September 2008
How to Cite
Masuho, I., Mototani, Y., Sahara, Y., Asami, J., Nakamura, S., Kozasa, T. and Inoue, T. (2008), Dynamic expression patterns of G protein-regulated inducer of neurite outgrowth 1 (GRIN1) and its colocalization with Gαo implicate significant roles of Gαo-GRIN1 signaling in nervous system. Dev. Dyn., 237: 2415–2429. doi: 10.1002/dvdy.21686
- Issue online: 26 AUG 2008
- Version of Record online: 26 AUG 2008
- Manuscript Accepted: 19 JUN 2008
- National Institutes of Health. Grant Numbers: GM61454, NS4144
- National Institute of Biomedical Innovation. Grant Number: 05-32
- gene expression;
- neural development;
GRIN1 (Gprin 1) is a signaling molecule coexpression of which with constitutively active form of Gαo can stimulate neurite extensions in Neuro2a cells, yet its in vivo roles remain elusive. Here, we examine expression profiles of GRIN1 during mouse development by in situ hybridization (ISH) and immunohistochemistry. ISH analysis revealed that GRIN1 expression was limited to the nervous system at all developmental stages tested: in the central nervous system, GRIN1 expression occurred within the entire embryonic mantle zones, while it became restricted to sets of nuclei at postnatal to adult stages. Immunohistochemistry using a GRIN1-specific antibody demonstrated that GRIN1 colocalized with Gαo at neuronal dendrites and axons, but it was not detected in glial cells. These results suggest that Gαo-GRIN1 pathway could mediate significant roles in neuronal migration and differentiation at embryonic stages and exert functions in wiring and/or maintenance of specific neural circuitries at postnatal to adult stages. Developmental Dynamics 237:2415–2429, 2008. © 2008 Wiley-Liss, Inc.