Enhancer detection and developmental expression of zebrafish sprouty1, a member of the fgf8 synexpression group

Authors

  • Anna Z. Komisarczuk,

    1. Sars International Centre for Marine Molecular Biology, Bergen High Technology Centre, Bergen, Norway
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  • Stefanie Topp,

    1. Zebrafish Neurogenetics Department, Institute of Developmental Genetics HelmholtzZentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany
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  • Christian Stigloher,

    1. Zebrafish Neurogenetics Department, Institute of Developmental Genetics HelmholtzZentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany
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  • Marika Kapsimali,

    1. INSERM U784 Genetique Moleculaire du Developpement, Ecole Normale Supérieure, Paris, France
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  • Laure Bally-Cuif,

    1. Zebrafish Neurogenetics Department, Institute of Developmental Genetics HelmholtzZentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany
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  • Thomas S. Becker

    Corresponding author
    1. Sars International Centre for Marine Molecular Biology, Bergen High Technology Centre, Bergen, Norway
    • Sars Centre for Marine Molecular Biology, University of Bergen, Thormøhlensgate 55, 5008 Bergen, Norway
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Abstract

Signaling pathways mediated by receptor tyrosine kinases (RTKs) are under positive and negative regulation, and misregulation of RTK signaling results in developmental defects and malignancy. A major class of antagonists of Fgf and Egf signaling are the Sprouty proteins. Through an enhancer detection approach, we isolated the sprouty1 (spry1) gene, expressed in multiple developing organs during embryogenesis. We analyzed expression of spry1 between tail bud stage and 10 days postfertilization. From the tail bud stage on, transcript and reporter are detected in the craniofacial region and in the mid–hindbrain boundary, where expression persists until adulthood. Further expression domains are the telencephalon, hindbrain, dorsal diencephalon and epiphysis, branchial arches, pituitary, and the tubular gill epithelium. In the trunk spry1 is also prominently expressed in pronephros, the lateral line and tail fin. Sprouty1 acts in Fgf signaling downstream of Fgfr1, as its expression is abrogated through the small molecule inhibitor of this receptor, SU5402. Developmental Dynamics 237:2594–2603, 2008. © 2008 Wiley-Liss, Inc.

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