C. Gentile and P.A. Fleming contributed equally to this work.
VEGF-mediated fusion in the generation of uniluminal vascular spheroids
Article first published online: 24 SEP 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on the Extracellular Matrix, in Memory of Dr. Elizabeth D. Hay
Volume 237, Issue 10, pages 2918–2925, October 2008
How to Cite
Gentile, C., Fleming, P. A., Mironov, V., Argraves, K. M., Argraves, W. S. and Drake, C. J. (2008), VEGF-mediated fusion in the generation of uniluminal vascular spheroids. Dev. Dyn., 237: 2918–2925. doi: 10.1002/dvdy.21720
- Issue published online: 24 SEP 2008
- Article first published online: 24 SEP 2008
- Manuscript Accepted: 29 JUL 2008
- NIH. Grant Numbers: HL57375, HL80168, HL061873, HL080404
- NS. Grant Number: FIBR-0526854
- vascular fusion;
- blood vessel;
- endothelial cell;
- smooth muscle cell
Embryonic mouse allantoic tissue (E8.5) was cultured in hanging drops to generate a three-dimensional vascular micro-tissue. The resulting tissue spheroids had an inner network of small diameter vessels expressing platelet endothelial cell adhesion molecule-1 (PECAM-1) and an outer layer of cells expressing SMαA, SM22-α, and SM-MHC. In a subsequent phase of culture, the fusion-promoting activity of vascular endothelial growth factor (VEGF) was used to transform the inner network of small diameter endothelial tubes into a contiguous layer of cells expressing PECAM-1, CD34, and VE-cadherin that circumscribed a central lumen-like cavity. The blood vessel-like character of the VEGF-treated spheroids was further demonstrated by their physiologically relevant vasodilatory and contractile responses, including contraction induced by KCl and relaxation stimulated by high-density lipoproteins and acetylcholine-induced nitric oxide production. Developmental Dynamics 237:2918–2925, 2008. © 2008 Wiley-Liss, Inc.