Analysis of the IKKβ/NF-κB signaling pathway during embryonic angiogenesis
Article first published online: 24 SEP 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on the Extracellular Matrix, in Memory of Dr. Elizabeth D. Hay
Volume 237, Issue 10, pages 2926–2935, October 2008
How to Cite
Hou, Y., Li, F., Karin, M. and Ostrowski, M. C. (2008), Analysis of the IKKβ/NF-κB signaling pathway during embryonic angiogenesis. Dev. Dyn., 237: 2926–2935. doi: 10.1002/dvdy.21723
- Issue published online: 24 SEP 2008
- Article first published online: 24 SEP 2008
- Manuscript Accepted: 29 JUL 2008
- NIH. Grant Numbers: R01 CA053271, P01 CA097189
- IKK (IκB kinase);
The nuclear factor-κB (NF-κB) signaling pathway regulates cellular growth, survival, differentiation and development. In this study, the functions of IκB kinase (IKK)β in angiogenesis during mouse development were examined. Conditional disruption of the Ikkβ locus in endothelial cells using the well-characterized Tie2-Cre transgene resulted in embryonic lethality between embryonic day (E) 13.5 and E15.5. Examination of the mutant embryos revealed that while deletion of Ikkβ occurred in endothelial cells throughout the embryo, only the vascular network in the fetal liver was affected. Disruption of the fetal liver vasculature was accompanied by decreased cell proliferation and increased apoptosis of hepatocytes, but hematopoiesis was not affected. Increased apoptosis was not observed outside of fetal liver in the mutant embryos. These results indicate that the IKKβ/NF-κB pathway plays a previously unappreciated role in development of the sinusoidal vasculature in the fetal liver and additionally that this pathway is critical in the crosstalk between endothelial cells and hepatocytes during mouse development. Developmental Dynamics 237:2926–2935, 2008. © 2008 Wiley-Liss, Inc.