A. Kwakowsky and M. Schwirtlich have contributed equally to this work.
Patterns & Phenotypes
GABA neurotransmitter signaling in the developing mouse lens: Dynamic regulation of components and functionality
Version of Record online: 4 NOV 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on Left-Right Asymmetry
Volume 237, Issue 12, pages 3830–3841, December 2008
How to Cite
Kwakowsky, A., Schwirtlich, M., Kooy, F., Ábrahám, I., Máté, Z., Katarova, Z. and Szabó, G. (2008), GABA neurotransmitter signaling in the developing mouse lens: Dynamic regulation of components and functionality. Dev. Dyn., 237: 3830–3841. doi: 10.1002/dvdy.21768
- Issue online: 20 NOV 2008
- Version of Record online: 4 NOV 2008
- Manuscript Accepted: 9 SEP 2008
- OTKA. Grant Numbers: T020638, T047217
- lens development;
- GABAA receptor subunits;
- GABAB receptor subunits;
- membrane GABA transporters (GAT1-4);
- vesicular GABA transporter (VGAT);
- non-neuronal GABA;
- intracellular calcium
Gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the vertebrate nervous system, serves as a signaling molecule modulating diverse processes during embryonic development. Earlier we have demonstrated that different forms of glutamic acid decarboxylase (GAD) are differentially regulated during mouse lens development. Here we show that the developing lens expresses also components of GABA signaling downstream of GAD. Multiple GABAA and GABAB receptor subunits as well as the GABA transporters show expression profiles highly correlated with the expression of different GADs. GABA receptors (GABAR) and the vesicular GABA transporter localize at the apical/basal membranes of the lens epithelia and differentiating fibers and may be involved in conventional GABAR-mediated signaling, while the membrane GABA transporters may also function as Na+/Cl−/GABA carriers. The functionality of GABAR was verified by calcium imaging in whole lenses. Our data suggest that GABA synthesized locally by GAD, acts through GABA receptors by modulating the intracellular calcium levels. Developmental Dynamics 237:3830–3841, 2008. © 2008 Wiley-Liss, Inc.