Epigenome and chromatin structure in human embryonic stem cells undergoing differentiation
Article first published online: 4 NOV 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on Left-Right Asymmetry
Volume 237, Issue 12, pages 3690–3702, December 2008
How to Cite
Bártová, E., Galiová, G., Krejčí, J., Harničarová, A., Strašák, L. and Kozubek, S. (2008), Epigenome and chromatin structure in human embryonic stem cells undergoing differentiation. Dev. Dyn., 237: 3690–3702. doi: 10.1002/dvdy.21773
- Issue published online: 20 NOV 2008
- Article first published online: 4 NOV 2008
- Manuscript Accepted: 10 SEP 2008
- Academy of Sciences of the Czech Republic. Grant Numbers: AVOZ50040507, IQS500040508
- The Grant Agency of the Czech Republic. Grant Number: 204/06/0978
- Ministry of Education, Youth and Sports of the Czech Republic. Grant Number: LC535
- histone modification;
Epigenetic histone (H3) modification patterns and the nuclear radial arrangement of select genetic elements were compared in human embryonic stem cells (hESCs) before and after differentiation. H3K9 acetylation, H3K9 trimethylation, and H3K79 monomethylation were reduced at the nuclear periphery of differentiated hESCs. Differentiation coincided with centromere redistribution, as evidenced by perinucleolar accumulation of the centromeric markers CENP-A and H3K9me3, central repositioning of centromeres 1, 5, 19, and rearrangement of other centromeres at the nuclear periphery. The radial positions of PML, RARα genes, and human chromosomes 10, 12, 15, 17, and 19 remained relatively stable as hESCs differentiated. However, the female inactive H3K27-trimethylated X chromosome occupied a more peripheral nuclear position in differentiated cells. Thus, pluripotent and differentiated hESCs have distinct nuclear patterns of heterochromatic structures (centromeres and inactive X chromosome) and epigenetic marks (H3K9me3, and H3K27me3), while relatively conserved gene density-related radial chromatin distributions are already largely established in undifferentiated hES cells. Developmental Dynamics 237:3690–3702, 2008. © 2008 Wiley-Liss, Inc.