Requirement for a core 1 galactosyltransferase in the Drosophila nervous system
Article first published online: 4 NOV 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on Left-Right Asymmetry
Volume 237, Issue 12, pages 3703–3714, December 2008
How to Cite
Lin, Y.-R., Reddy, B.V.V.G. and Irvine, K. D. (2008), Requirement for a core 1 galactosyltransferase in the Drosophila nervous system. Dev. Dyn., 237: 3703–3714. doi: 10.1002/dvdy.21775
- Issue published online: 20 NOV 2008
- Article first published online: 4 NOV 2008
- Manuscript Accepted: 12 SEP 2008
- The Mizutani Foundation for Glycoscience. Grant Number: 060029
- Howard Hughes Medical Institute
- nervous system
Mucin type O-glycosylation is a widespread modification of eukaryotic proteins, but its functional requirements remain incompletely understood. It is initiated by the attachment of N-acetylgalactosamine (GalNAc) to Ser or Thr residues, and then elongated by additional sugars. We have examined requirements for mucin-type glycosylation in Drosophila by characterizing the expression and phenotypes of core 1 galactosyltransferases (core 1 GalTs), which elongate O-GalNAc by adding galactose in a β1,3 linkage. Drosophila encode several putative core 1 GalTs, each expressed in distinct patterns. CG9520 (C1GalTA) is expressed in the amnioserosa and central nervous system. A null mutation in C1GalTA is lethal, and mutant animals exhibit a striking morphogenetic defect in which the ventral nerve cord is greatly elongated and the brain hemispheres are misshapen. Lectin staining and blotting experiments confirmed that C1GalTA contributes to the synthesis of Gal-β1,3-GalNAc in vivo. Our results identify a role for mucin-type O-glycosylation during neural development in Drosophila. Developmental Dynamics 237:3703–3714, 2008. © 2008 Wiley-Liss, Inc.