Dnm3os, a non-coding RNA, is required for normal growth and skeletal development in mice
Version of Record online: 4 NOV 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on Left-Right Asymmetry
Volume 237, Issue 12, pages 3738–3748, December 2008
How to Cite
Watanabe, T., Sato, T., Amano, T., Kawamura, Y., Kawamura, N., Kawaguchi, H., Yamashita, N., Kurihara, H. and Nakaoka, T. (2008), Dnm3os, a non-coding RNA, is required for normal growth and skeletal development in mice. Dev. Dyn., 237: 3738–3748. doi: 10.1002/dvdy.21787
- Issue online: 20 NOV 2008
- Version of Record online: 4 NOV 2008
- Manuscript Accepted: 14 SEP 2008
- Ministry of Education, Culture, Sports, Science and Technology. Grant Number: 18790703
- Japan Heart Foundation Research
- non-coding RNA;
- micro RNA;
- skeletal development
Dnm3os, a gene that is transcribed into a non-coding RNA (ncRNA), contains three micro RNAs (miRNAs), miR-199a, miR-199a*, and miR-214, whose functions remain unknown in mammals. In this study, we introduced the lacZ gene into the Dnm3os locus to recapitulate its expression pattern and disrupt its function. Dnm3os+/lacZ heterozygous embryos showed β-galactosidase activity, which reflected the authentic expression pattern of Dnm3os RNA. Most of the Dnm3oslacZ/lacZ homozygous pups died within one month of birth. After birth, Dnm3oslacZ/lacZ mice exhibited several skeletal abnormalities, including craniofacial hypoplasia, defects in dorsal neural arches and spinous processes of the vertebrae, and osteopenia. Importantly, the expression of miR-199a, miR-199a*, and miR-214 was significantly down-regulated in Dnm3oslacZ/lacZ embryos, supporting the assumption that Dnm3os serves as a precursor of these three miRNAs. Thus, Dnm3os has emerged as an miRNA-encoding gene that is indispensable for normal skeletal development and body growth in mammals. Developmental Dynamics 237:3738–3748, 2008. © 2008 Wiley-Liss, Inc.