pMesogenin1 and 2 function directly downstream of Xtbx6 in Xenopus somitogenesis and myogenesis
Article first published online: 25 NOV 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on Left-Right Asymmetry
Volume 237, Issue 12, pages 3749–3761, December 2008
How to Cite
Tazumi, S., Yabe, S., Yokoyama, J., Aihara, Y. and Uchiyama, H. (2008), pMesogenin1 and 2 function directly downstream of Xtbx6 in Xenopus somitogenesis and myogenesis. Dev. Dyn., 237: 3749–3761. doi: 10.1002/dvdy.21791
- Issue published online: 25 NOV 2008
- Article first published online: 25 NOV 2008
- Manuscript Accepted: 27 SEP 2008
- 2007 Strategic Research Project of Yokohama City University, Japan. Grant Number: K19007
- body wall muscle;
T-box transcription factor tbx6 and basic-helix-loop-helix transcription factor pMesogenin1 are reported to be involved in paraxial mesodermal differentiation. To clarify the relationship between these genes in Xenopus laevis, we isolated pMesogenin2, which showed high homology with pMesogenin1. Both pMesogenin1 and 2 appeared to be transcriptional activators and were induced by a hormone-inducible version of Xtbx6 without secondary protein synthesis in animal cap assays. The pMesogenin2 promoter contained three potential T-box binding sites with which Xtbx6 protein was shown to interact, and a reporter gene construct containing these sites was activated by Xtbx6. Xtbx6 knockdown reduced pMesogenin1 and 2 expressions, but not vice versa. Xtbx6 and pMesogenin1 and 2 knockdowns caused similar phenotypic abnormalities including somite malformation and ventral body wall muscle hypoplasia, suggesting that Xtbx6 is a direct regulator of pMesogenin1 and 2, which are both involved in somitogenesis and myogenesis including that of body wall muscle in Xenopus laevis. Developmental Dynamics 237:3749–3761, 2008. © 2008 Wiley-Liss, Inc.