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dvdy_21792_sm_SuppFigS1.tif652KFig. S1. Genomic structure of the bus-1 gene with the position of identified mutations. The position of the region that codes for sequence homology domain acyltransferase 3 family is indicated. The Tc1 insertions in e2746, e2720, e2751, and e2738 were at 261, 993, 1,404, and 1,545 bp from the predicted ATG, respectively; the Tc3 insertions in e2757 and e2743 were mapped to the corresponding positions 531 and 1,392; the C >T transitions in e2683 and e2687 were at positions 68 and 169; the G >A transition in e2712 was at position 587.
dvdy_21792_sm_SuppFigS2.tif2661KFig. S2. Phylogenetic analysis of the Acyltransferase 3 family genes in C. elegans. The phylogenetic tree was constructed with the acyltransferase domain of the 65 members in the C. elegans genome from a distance matrix created with the Jukes-Cantor method using neighbour-joining method.
dvdy_21792_sm_SuppFigS3.tif2353KFig. S3. Alignment of bus-1 upstream region from C. elegans and C. briggsae identifies conserved sequence blocks. The sequence ends with the predicted start codon for Cel bus-1 and C. briggsae CBG19162. Asterisk indicates the stop of the previous gene in C. elegans (R03H4.7) and C. briggsae (CBG19163). A putative MAB-3 DSX binding site in boxed. Identical residues are highlighted in black and conserved residues in gray.
dvdy_21792_sm_SuppFigS4.tif1693KFig. S4. Hypersensitivity of mab-9(e2410) males to M. nematophilum. A: Infected mab-9 male, SYTO13 stained, showing bacterial colonization inside the body cavity in the tail region. B: Degeneration of male tail. C: Disintegration of male tail. D: The double mab-9;bus-1 exhibits no swelling on OP50, but animals are Dar when exposed to M. nematophilum (E). Arrow points to bacterially colonized rectum.
dvdy_21792_sm_SuppFigS5.tif1087KFig. S5. Expression of oac-41/R03H4.5 in dauer larva seam cells. A (merged) and B (fluorescent) images of animals carrying eEx601, with the promoter region of oac-41 driving DsRed2.
dvdy_21792_sm_SuppTableS1.doc81KTable S1. C. elegans mutations/RNAi in genes associated with rectal epithelial cells/rectum, their response to M. nematophilum and bus-1 expression.

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