The acyltransferase gene bus-1 exhibits conserved and specific expression in nematode rectal cells and reveals pathogen-induced cell swelling
Article first published online: 25 NOV 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on Left-Right Asymmetry
Volume 237, Issue 12, pages 3762–3776, December 2008
How to Cite
Gravato-Nobre, M. J. and Hodgkin, J. (2008), The acyltransferase gene bus-1 exhibits conserved and specific expression in nematode rectal cells and reveals pathogen-induced cell swelling. Dev. Dyn., 237: 3762–3776. doi: 10.1002/dvdy.21792
- Issue published online: 25 NOV 2008
- Article first published online: 25 NOV 2008
- Manuscript Accepted: 1 OCT 2008
- Medical Research Council, UK
- NIH National Center for Resources (NCRR)
- C. elegans Gene Knockout Consortium
- National BioResource Project, Tokyo, Japan
Additional supporting information may be found in the online version of this article.
|dvdy_21792_sm_SuppFigS1.tif||652K||Fig. S1. Genomic structure of the bus-1 gene with the position of identified mutations. The position of the region that codes for sequence homology domain acyltransferase 3 family is indicated. The Tc1 insertions in e2746, e2720, e2751, and e2738 were at 261, 993, 1,404, and 1,545 bp from the predicted ATG, respectively; the Tc3 insertions in e2757 and e2743 were mapped to the corresponding positions 531 and 1,392; the C >T transitions in e2683 and e2687 were at positions 68 and 169; the G >A transition in e2712 was at position 587.|
|dvdy_21792_sm_SuppFigS2.tif||2661K||Fig. S2. Phylogenetic analysis of the Acyltransferase 3 family genes in C. elegans. The phylogenetic tree was constructed with the acyltransferase domain of the 65 members in the C. elegans genome from a distance matrix created with the Jukes-Cantor method using neighbour-joining method.|
|dvdy_21792_sm_SuppFigS3.tif||2353K||Fig. S3. Alignment of bus-1 upstream region from C. elegans and C. briggsae identifies conserved sequence blocks. The sequence ends with the predicted start codon for Cel bus-1 and C. briggsae CBG19162. Asterisk indicates the stop of the previous gene in C. elegans (R03H4.7) and C. briggsae (CBG19163). A putative MAB-3 DSX binding site in boxed. Identical residues are highlighted in black and conserved residues in gray.|
|dvdy_21792_sm_SuppFigS4.tif||1693K||Fig. S4. Hypersensitivity of mab-9(e2410) males to M. nematophilum. A: Infected mab-9 male, SYTO13 stained, showing bacterial colonization inside the body cavity in the tail region. B: Degeneration of male tail. C: Disintegration of male tail. D: The double mab-9;bus-1 exhibits no swelling on OP50, but animals are Dar when exposed to M. nematophilum (E). Arrow points to bacterially colonized rectum.|
|dvdy_21792_sm_SuppFigS5.tif||1087K||Fig. S5. Expression of oac-41/R03H4.5 in dauer larva seam cells. A (merged) and B (fluorescent) images of animals carrying eEx601, with the promoter region of oac-41 driving DsRed2.|
|dvdy_21792_sm_SuppTableS1.doc||81K||Table S1. C. elegans mutations/RNAi in genes associated with rectal epithelial cells/rectum, their response to M. nematophilum and bus-1 expression.|
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