Patterns & Phenotypes
Caspase-2L, caspase-9, and caspase-3 during in vitro maturation and fragmentation of the mouse oocyte
Article first published online: 25 NOV 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on Left-Right Asymmetry
Volume 237, Issue 12, pages 3892–3903, December 2008
How to Cite
Arnault, E., Tosca, L., Courtot, A.-M., Doussau, M., Pesty, A., Finaz, C., Allemand, I. and Lefèvre, B. (2008), Caspase-2L, caspase-9, and caspase-3 during in vitro maturation and fragmentation of the mouse oocyte. Dev. Dyn., 237: 3892–3903. doi: 10.1002/dvdy.21793
- Issue published online: 25 NOV 2008
- Article first published online: 25 NOV 2008
- Manuscript Accepted: 1 OCT 2008
Several studies have shown that apoptotic pathways control fragmentation of unfertilized ovulated oocyte, induced by doxorubicin. But very few have investigated the basis of this process, from prophase I to later stages. Our results revealed the presence of caspase-2L, caspase-9, and caspase-3 in their zymogen and cleaved forms in the oocyte before meiosis resumption. Caspase-2L and caspase-9 were detected in the nucleus of GV-oocytes in a distribution related to chromatin configuration. The inhibition of caspase activity by Z-VAD-fmk accelerated the transition from metaphase I to metaphase II, and caspase-9 and caspase-3 were detected along the meiotic spindle. Surprisingly, Western blot analysis revealed that the three cleaved caspases were present in similar amounts in healthy and fragmented oocytes and caspase inhibition did not prevent doxorubicin-induced apoptosis. Our results suggest that, if cleaved, caspases may be dispensable for final oocyte death and they could be involved in regulating the maturation process. Developmental Dynamics 237:3892–3903, 2008. © 2008 Wiley-Liss, Inc.