The lim domain only protein 7 is important in zebrafish heart development

Authors

  • Elisabeth B. Ott,

    1. Institute of Biology, Department of Molecular and Cellular Biology, University of Leiden, Leiden, The Netherlands
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  • Nynke M. S. van den Akker,

    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    2. Department of Cardiology, Cardiovascular Research Institute Maastricht, University Maastricht, Maastricht, The Netherlands
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  • Philippe A. Sakalis,

    1. Institute of Biology: Department of Integrative Zoology University of Leiden, Leiden, The Netherlands
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  • Adriana C. Gittenberger-de Groot,

    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
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  • Aartjan J.W. Te Velthuis,

    1. Institute of Biology, Department of Molecular and Cellular Biology, University of Leiden, Leiden, The Netherlands
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  • Christoph P. Bagowski

    Corresponding author
    1. Institute of Biology, Department of Molecular and Cellular Biology, University of Leiden, Leiden, The Netherlands
    2. Institute of Biology: Department of Integrative Zoology University of Leiden, Leiden, The Netherlands
    • Institute of Biology, University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
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Abstract

The LIM domain only protein 7 (LMO7), a member of the PDZ and LIM domain-containing protein family is a candidate gene with possible roles in embryonic development and breast cancer progression. LMO7 has been linked to actin cytoskeleton organization through nectin/afadin and to cell–cell adhesion by means of E-cadherin/catenin. In addition, LMO7 has been shown to regulate transcription of the nuclear membrane protein Emerin and other muscle relevant genes. In this study, we used in situ hybridization to investigate LMO7 expression during embryonic development in three widely used vertebrate model species: the zebrafish, the chicken and the mouse. Our temporal and spatial gene expression analysis revealed both common and distinct patterns between these species. In mouse and chicken embryos we found expression in the outflow tract, the inflow tract, the pro-epicardial organ and the second heart field, structures highly important in the developing heart. Furthermore, gene knockdown experiments in zebrafish embryos resulted in severe defects in heart development with effects on the conduction system and on heart localization. In summary, we present here the first developmental study of LMO7. We reveal the temporal and spatial expression patterns of this important gene during mouse, chicken and fish development and our findings suggest essential functions for LMO7 during vertebrate heart development. Developmental Dynamics 237:3940–3952, 2008. © 2008 Wiley-Liss, Inc.

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