Many ribosomal protein mutations are associated with growth impairment and tumor predisposition in zebrafish
Version of Record online: 18 DEC 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 238, Issue 1, pages 76–85, January 2009
How to Cite
Lai, K., Amsterdam, A., Farrington, S., Bronson, R. T., Hopkins, N. and Lees, J. A. (2009), Many ribosomal protein mutations are associated with growth impairment and tumor predisposition in zebrafish. Dev. Dyn., 238: 76–85. doi: 10.1002/dvdy.21815
- Issue online: 18 DEC 2008
- Version of Record online: 18 DEC 2008
- Manuscript Accepted: 23 OCT 2008
- NIH-NCI. Grant Number: CA106416
Additional Supporting Information may be found in the online version of this article.
|dvdy_21815_sm_SupFigS1.tif||540K||Fig. S1. Polysome profiles do not reveal an overt translation defect in hi258 (rpL35) heterozygous embryos. A: Polysome profile generated from an embryo mixture enriched for wild-type embryos. Peaks representing the small ribosomal subunit (40S), the large ribosomal subunit (60S), the monosomes (80S), and the polysomes are detected. B: Polysome profile of an embryo mixture enriched for hi258 (rpL35) heterozygotes appears very similar to that of the wild-type-enriched embryo mixture. C: Homozygous mutants for hi258 (rpL35) display a general reduction in all peaks. The decrease of the 60S peak is relatively most severe. D: Homozygous mutants for hi1974 (rpS8) display a loss of the 40S peak, a relative increase in the 60S peak, and a reduction in the 80S and polysome peaks.|
|dvdy_21815_sm_SupTabsS1-S2.docx||12K||Supplemental Tables S1–S2|
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