Patterns & Phenotypes
Cloning and expression analysis of the anterior parahox genes, Gsh1 and Gsh2 from Xenopus tropicalis
Article first published online: 18 DEC 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 238, Issue 1, pages 194–203, January 2009
How to Cite
Illes, J. C., Winterbottom, E. and Isaacs, H. V. (2009), Cloning and expression analysis of the anterior parahox genes, Gsh1 and Gsh2 from Xenopus tropicalis. Dev. Dyn., 238: 194–203. doi: 10.1002/dvdy.21816
- Issue published online: 18 DEC 2008
- Article first published online: 18 DEC 2008
- Manuscript Accepted: 26 OCT 2008
- Biotechnology and Biological Sciences Research Council. Grant Numbers: BBSSC200212885, BBSSC200513195
Additional Supporting Information may be found in the online version of this article.
|dvdy_21816_sm_SuppFigsS1.tif||4354K||Supp. Fig. 1. Clustal W alignments of Xenopus tropicalis Gsh1 and Gsh2 proteins. Peptide sequences for Xenopus tropicalis Gsh1 (A) and Gsh2 (B), aligned with Gsx orthologues from a number of species using the Clustal W method. Asterisks below the alignment mark the HEP motif; the homeodomain is indicated by chevrons beneath the alignment. Percentage identity of each protein to the relevant X. tropicalis sequence is indicated.|
|dvdy_21816_sm_SuppFigsS2.tif||179K||Supp. Fig. 2. Phylogenetic tree of Gsx proteins. Phylogentic tree produced in Jalview (Clamp et al., 2004), using the neighor joining method with %. Xt, Xenopus tropicalis; Ol, Oryzias latipes; Mm, Mus musculus; Dm, Drosophila melanogaster.|
|dvdy_21816_sm_SuppFigsS3.tif||1404K||Supp. Fig. 3. Gsh1 and Gsh2 expression in the tailbud stage X. tropicalis brain. Diagram of a stage 31–32 X. tropicalis brain, viewed laterally, showing the expression patterns of Gsh1 (magenta) and Gsh2 (blue). Hatching indicates overlap of the expression patterns. di, diencephalon; et, eminentia thalami; hyp, hypothalamus; lge, lateral ganglionic eminence; mes, mesencephalon; mge, medial ganglionic eminence; nc, notochord; ob, olfactory bulb; os, optic stalk; ov, otic vesicle; pa, pallium; pi, pineal gland; pt, pretectum; pth, prethalamus; r1–r8, rhombomeres 1–8; rhomb, rhombencephalon; tec, tectum; sc, spinal cord; tel, telencephalon; th, thalamus; zli, zona limitans intrathalamica.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.