Additional Supporting Information may be found in the online version of this article.

dvdy_21816_sm_SuppFigsS1.tif4354KSupp. Fig. 1. Clustal W alignments of Xenopus tropicalis Gsh1 and Gsh2 proteins. Peptide sequences for Xenopus tropicalis Gsh1 (A) and Gsh2 (B), aligned with Gsx orthologues from a number of species using the Clustal W method. Asterisks below the alignment mark the HEP motif; the homeodomain is indicated by chevrons beneath the alignment. Percentage identity of each protein to the relevant X. tropicalis sequence is indicated.
dvdy_21816_sm_SuppFigsS2.tif179KSupp. Fig. 2. Phylogenetic tree of Gsx proteins. Phylogentic tree produced in Jalview (Clamp et al., 2004), using the neighor joining method with %. Xt, Xenopus tropicalis; Ol, Oryzias latipes; Mm, Mus musculus; Dm, Drosophila melanogaster.
dvdy_21816_sm_SuppFigsS3.tif1404KSupp. Fig. 3. Gsh1 and Gsh2 expression in the tailbud stage X. tropicalis brain. Diagram of a stage 31–32 X. tropicalis brain, viewed laterally, showing the expression patterns of Gsh1 (magenta) and Gsh2 (blue). Hatching indicates overlap of the expression patterns. di, diencephalon; et, eminentia thalami; hyp, hypothalamus; lge, lateral ganglionic eminence; mes, mesencephalon; mge, medial ganglionic eminence; nc, notochord; ob, olfactory bulb; os, optic stalk; ov, otic vesicle; pa, pallium; pi, pineal gland; pt, pretectum; pth, prethalamus; r1–r8, rhombomeres 1–8; rhomb, rhombencephalon; tec, tectum; sc, spinal cord; tel, telencephalon; th, thalamus; zli, zona limitans intrathalamica.

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