Additional Supporting Information may be found in the online version of this article.

dvdy_21830_sm_SupFigS1.tif1553KSupp. Fig. S1. High Evolutionary conservation of Frg1. The alignment of consensus human, Caenorhabditis elegans and Xenopus Frg1 amino acid sequences.
dvdy_21830_sm_SupFigS2.tif7660KSupp. Fig. S2. FRG1 protein expression is becomes restricted. A–H: Stage 30 embryos were sectioned sagittally (A,B,E,F) or transversely (C,D,G,H) and either immunostained for FRG1 (A–D) or DAPI (4′,6-diamidine-2-phenylidole-dihydrochloride ) stained. Arrowheads point to one example of a nuclei dense region that is devoid of FRG1 staining.
dvdy_21830_sm_SupFigS3.tif12298KSupp. Fig. S3. Overexpression and knock down of frg1 has no effect on NCAM expression. A,B: Right side of embryos injected with 1 ng of Frg1 mRNA compared with left side uninjected, respectively. C: View of right side of embryos injected with 40 ng of frg1 morpholino on the right side. D: Dorsal view of embryos in C, showing equivalent staining on injected (right) and uninjected sides (left)
dvdy_21830_sm_SupFigS4.tif2198KSupp. Fig. S4. XLFRG1 antibody Western blot of St. 42 embryo extract. XLFRG1 antibody recognizes a single band at the predicted molecular weight for Xenopus laevis FRG1 of 30 kDa.
dvdy_21830_sm_SupTableS1.docx11KSupplementary Table S1. Primer Sequences

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