Drs. Hanel and Wuebbles contributed equally to this work.
Special Issue Disease Connections
Muscular dystrophy candidate gene FRG1 is critical for muscle development
Version of Record online: 18 DEC 2008
Copyright © 2008 Wiley-Liss, Inc.
Special Issue: Special Focus on Xenopus
Volume 238, Issue 6, pages 1502–1512, June 2009
How to Cite
Hanel, M. L., Wuebbles, R. D. and Jones, P. L. (2009), Muscular dystrophy candidate gene FRG1 is critical for muscle development. Dev. Dyn., 238: 1502–1512. doi: 10.1002/dvdy.21830
- Issue online: 12 MAY 2009
- Version of Record online: 18 DEC 2008
- Manuscript Accepted: 3 NOV 2008
- NIH/NIAMS. Grant Number: 1RO1AR055877
- FSH Society Landsman Charitable Trust Fellowship. Grant Number: FSHS-LCT-001
Additional Supporting Information may be found in the online version of this article.
|dvdy_21830_sm_SupFigS1.tif||1553K||Supp. Fig. S1. High Evolutionary conservation of Frg1. The alignment of consensus human, Caenorhabditis elegans and Xenopus Frg1 amino acid sequences.|
|dvdy_21830_sm_SupFigS2.tif||7660K||Supp. Fig. S2. FRG1 protein expression is becomes restricted. A–H: Stage 30 embryos were sectioned sagittally (A,B,E,F) or transversely (C,D,G,H) and either immunostained for FRG1 (A–D) or DAPI (4′,6-diamidine-2-phenylidole-dihydrochloride ) stained. Arrowheads point to one example of a nuclei dense region that is devoid of FRG1 staining.|
|dvdy_21830_sm_SupFigS3.tif||12298K||Supp. Fig. S3. Overexpression and knock down of frg1 has no effect on NCAM expression. A,B: Right side of embryos injected with 1 ng of Frg1 mRNA compared with left side uninjected, respectively. C: View of right side of embryos injected with 40 ng of frg1 morpholino on the right side. D: Dorsal view of embryos in C, showing equivalent staining on injected (right) and uninjected sides (left)|
|dvdy_21830_sm_SupFigS4.tif||2198K||Supp. Fig. S4. XLFRG1 antibody Western blot of St. 42 embryo extract. XLFRG1 antibody recognizes a single band at the predicted molecular weight for Xenopus laevis FRG1 of 30 kDa.|
|dvdy_21830_sm_SupTableS1.docx||11K||Supplementary Table S1. Primer Sequences|
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