Analysis of human muscle stem cells reveals a differentiation-resistant progenitor cell population expressing Pax7 capable of self-renewal

Authors

  • Bradley Pawlikowski,

    Corresponding author
    1. Department of Cell and Tissue Biology, University of California San Francisco, School of Dentistry, San Francisco, California
    • University of California, San Francisco, Department of Cell and Tissue Biology, 521 Parnassus Avenue, Box 0640, San Francisco, CA 94143
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  • Laimeng Lee,

    1. Department of Cell and Tissue Biology, University of California San Francisco, School of Dentistry, San Francisco, California
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  • Jianhong Zuo,

    1. Department of Cell and Tissue Biology, University of California San Francisco, School of Dentistry, San Francisco, California
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  • Randall H. Kramer

    1. Department of Cell and Tissue Biology, University of California San Francisco, School of Dentistry, San Francisco, California
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Abstract

Studies using mouse models have established a critical role for resident satellite stem cells in skeletal muscle development and regeneration, but little is known about this paradigm in human muscle. Here, using human muscle stem cells, we address their lineage progression, differentiation, migration, and self-renewal. Isolated human satellite cells expressed α7-integrin and other definitive muscle markers, were highly motile on laminin substrates and could undergo efficient myotube differentiation and myofibrillogenesis. However, only a subpopulation of the myoblasts expressed Pax7 and displayed a variable lineage progression as measured by desmin and MyoD expression. Analysis identified a differentiation-resistant progenitor cell population that was Pax7+/desmin and capable of self-renewal. This study extends our understanding of the role of Pax7 in regulating human satellite stem cell differentiation and self-renewal. Developmental Dynamics 238:138–149, 2009. © 2008 Wiley-Liss, Inc.

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