Analysis of human muscle stem cells reveals a differentiation-resistant progenitor cell population expressing Pax7 capable of self-renewal
Version of Record online: 18 DEC 2008
Copyright © 2008 Wiley-Liss, Inc.
Volume 238, Issue 1, pages 138–149, January 2009
How to Cite
Pawlikowski, B., Lee, L., Zuo, J. and Kramer, R. H. (2009), Analysis of human muscle stem cells reveals a differentiation-resistant progenitor cell population expressing Pax7 capable of self-renewal. Dev. Dyn., 238: 138–149. doi: 10.1002/dvdy.21833
- Issue online: 18 DEC 2008
- Version of Record online: 18 DEC 2008
- Manuscript Accepted: 15 NOV 2008
- NIH. Grant Number: R01 DE 015404
Additional Supporting Information may be found in the online version of this article.
|dvdy_21833_sm_SuppFigS1.tif||2776K||Supp. Fig. S1. Heterogeneous expression of Pax7, MyoD, and desmin by adult α7 integrin-sorted human cultured cells. A: Immunostaining for c-Met/desmin shows cells that are positive for both (arrow) and cells that express c-Met but not desmin (arrowhead). B: Immunostaining for MyoD/desmin shows cells that are positive for both (arrow) and cells that express desmin but not MyoD (arrowhead). C: Immunostaining for Pax7 and desmin on adult muscle cultures grown in DM for 7–10 days to induce myotube formation demonstrated that some cells in the differentiation-resistant population are Pax7+/desmin+ (arrow), while others are Pax7+/desmin− (arrowhead). Scale bar = 20 μM.|
|dvdy_21833_sm_SuppFigS2.tif||2790K||Supp. Fig. S2. Homogeneous expression of Pax7, MyoD, and desmin by cultured mouse myoblasts. A–C: Immunostaining of mouse primary myoblasts for MyoD (A), Desmin (B), Pax7 (C) show uniform expression by all cells for these proteins.|
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