The homeobox gene Mohawk represses transcription by recruiting the sin3A/HDAC co-repressor complex
Article first published online: 22 FEB 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 3, pages 572–580, March 2009
How to Cite
Anderson, D. M., Beres, B. J., Wilson-Rawls, J. and Rawls, A. (2009), The homeobox gene Mohawk represses transcription by recruiting the sin3A/HDAC co-repressor complex. Dev. Dyn., 238: 572–580. doi: 10.1002/dvdy.21873
- Issue published online: 22 FEB 2009
- Article first published online: 22 FEB 2009
- Manuscript Accepted: 18 DEC 2008
Mohawk is an atypical homeobox gene expressed in embryonic progenitor cells of skeletal muscle, tendon, and cartilage. We demonstrate that Mohawk functions as a transcriptional repressor capable of blocking the myogenic conversion of 10T1/2 fibroblasts. The repressor activity is located in three small, evolutionarily conserved domains (MRD1–3) in the carboxy-terminal half of the protein. Point mutation analysis revealed six residues in MRD1 are sufficient for repressor function. The carboxy-terminal half of Mohawk is able to recruit components of the Sin3A/HDAC co-repressor complex (Sin3A, Hdac1, and Sap18) and a subset of Polymerase II general transcription factors (Tbp, TFIIA1 and TFIIB). Furthermore, Sap18, a protein that bridges the Sin3A/HDAC complex to DNA-bound transcription factors, is co-immunoprecipitated by MRD1. These data predict that Mohawk can repress transcription through recruitment of the Sin3A/HDAC co-repressor complex, and as a result, repress target genes required for the differentiation of cells to the myogenic lineage. Developmental Dynamics 238:572–580, 2009. © 2009 Wiley-Liss, Inc.