Notch signaling downstream of foxD5 promotes neural ectodermal transcription factors that inhibit neural differentiation

Authors

  • Bo Yan,

    1. Department of Anatomy and Regenerative Biology, The George Washington University Medical Center, Washington, DC
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  • Karen M. Neilson,

    1. Department of Anatomy and Regenerative Biology, The George Washington University Medical Center, Washington, DC
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  • Sally A. Moody

    Corresponding author
    1. Department of Anatomy and Regenerative Biology, The George Washington University Medical Center, Washington, DC
    • Department of Anatomy and Regenerative Biology, The George Washington University Medical Center, 2300 I (eye) Street, N.W., Washington, DC 20037
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Abstract

We investigated the role of the Notch signaling pathway in regulating several transcription factors that stabilize a neural fate and expand the neural plate. Increased Notch signaling in a neural lineage via a constitutively activated form (NICD) up-regulated geminin and zic2 in a cell-autonomous manner, and expanded the neural plate domains of sox11, sox2, and sox3. Loss- and gain-of-function assays show that foxD5 acts upstream of notch1 gene expression. Decreasing Notch signaling with an anti-morphic form of a Notch ligand (X-Delta-1STU) showed that the foxD5-mediated expansion of the sox gene neural plate domains requires Notch signaling. However, geminin and zic2 appear to be dually regulated by foxD5 and Notch1 signaling. These studies demonstrate that: (1) Notch signaling acts downstream of foxD5 to promote the expression of a subset of neural ectodermal transcription factors; and (2) Notch signaling and the foxD5 transcriptional pathway together maintain the neural plate in an undifferentiated state. Developmental Dynamics 238:1358–1365, 2009. © 2009 Wiley-Liss, Inc.

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