Novel markers of early ovarian pre-granulosa cells are expressed in an Sry-like pattern
Article first published online: 19 MAR 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 4, pages 812–825, April 2009
How to Cite
Lee, H. J., Pazin, D. E., Kahlon, R. S., Correa, S. M. and Albrecht, K. H. (2009), Novel markers of early ovarian pre-granulosa cells are expressed in an Sry-like pattern. Dev. Dyn., 238: 812–825. doi: 10.1002/dvdy.21902
- Issue published online: 19 MAR 2009
- Article first published online: 19 MAR 2009
- Manuscript Accepted: 5 JAN 2009
- Department of Medicine, BUSM (Pilot Project)
- National Institute of Child Health and Human Development. Grant Number: HD042779
- American Cancer Society Research Scholar Grant. Grant Number: 06-033-01-DDC
- sex determination;
- gene expression;
Mammalian gonad differentiation involves sexually dimorphic cell-fate decisions within the bipotential gonadal primordia. Testis differentiation is initiated by a center-to-poles wave of Sry expression that induces supporting cell precursors (SCPs) to become Sertoli rather than granulosa cells. The initiation of ovary differentiation is less well understood. We identified two novel SCP markers, 1700106J16Rik and Sprr2d, whose expression is ovary-biased during early gonad development, and altered in Wnt4, Sf1, Wt1, and Fog2 mutant gonads. In XX and XY gonads, both genes were up-regulated at ∼E11 in a center-to-poles wave, and then rapidly down-regulated in XY gonads in a center-to-poles wave, which is reminiscent of Sry expression in XY gonads. Our data suggest that 1700106J16Rik and Sprr2d may have important roles in early gonad development, and are consistent with the hypothesis that ovarian SCP differentiation occurs in a center-to-poles wave with similar timing to that of testicular SCP differentiation. Developmental Dynamics 238:812–825, 2009. © 2009 Wiley-Liss, Inc.