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DVDY_21913_sm_SupplFigS1.tif550KSupp. Fig. S1. Analysis of the genomic locus surrounding X. tropicalis fgf genes. A: Synteny of fgf 2, fgf3, fgf4, fgf19, fgf6, and fgf23 were analysed for human, mouse, and Xenopus tropicalis. Chromosomal loci were constructed from Metazome (http://www.metazome.net/) showing the high level of conservation of the gene loci surrounding fgf genes. For each alignment, boxes with the same colour correspond to the same gene; white boxes correspond to genes without annotation or without orthologues in the species shown here. Hsa = Homo sapiens; Mmu = Mus musculus; and Xtr = Xenopus tropicalis. B: Identification of a partial sequence of fgf21. By synteny analysis, we have identified the region corresponding to the fgf21 locus in Xenopus tropicalis. We then used an EST for Xenopus laevis fgf21 (accession number EB470635) to identify the sequence in the X. tropicalis genome. This region of the genome contains numerous gaps (black boxes), which probably explain why we were unable to identify the full sequence. C: Identification of the genomic locus where fgf17 would be expected to be located. Our efforts to find X. tropicalis fgf17 were unsuccessful despite a fairly complete sequence around this region of the genome. Hsa = Homo sapiens; Mmu = Mus musculus; Xtr = Xenopus tropicalis.
DVDY_21913_sm_SupplFigS2.tif137KSupp. Fig. S2. Phylogenetic tree of fgf sequences. Unrooted N-J phylogenetic tree of fgf sequences using CLUSTALW (http://align.genome.jp/) showing the subdivision of the different fgfs into seven subfamilies in Xenopus tropicalis.
DVDY_21913_sm_SupplFigS3.tif733KSupp. Fig. S3. Schematic representation of a Xenopus embryo at stage 28. A schematic representation of a stage-28 embryo with the different tissues coloured and annotated. The yellow-green area at the very anterior of the embryo includes the forebrain, anterior mesoderm, and the anterior placode area. Just posterior in brown is the olfactory placodes. The eye is in purple with the lens in dark purple. The trigeminal nerve is in pink and the otic vesicle in orange. The grey area represents the branchial arches. The neural tube is yellow with the Midbrain-Hindbrain Boundary in dark pink. The notochord in red runs ventral to the neural tube. The light blue stripes represent the somites and the blue area posterior to the somites is the presomitic mesoderm. The tailbud is in dark blue. The pronephric tubules and duct are in burgundy. The fin core is in dark green while the fin crest is in light green.
DVDY_21913_sm_SuppTablesS1-S2.doc167KSupplementary Table 1: Oligonucleotides used for the RT-PCR experiments. All oligonucleotides are written from 5′ to 3′; Supplementary Table 2: Origin and accession number of the clones used for the in situ analyses.

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