Periostin promotes a fibroblastic lineage pathway in atrioventricular valve progenitor cells
Article first published online: 30 MAR 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 5, pages 1052–1063, May 2009
How to Cite
Norris, R. A., Potts, J. D., Yost, M. J., Junor, L., Brooks, T., Tan, H., Hoffman, S., Hart, M. M., Kern, M. J., Damon, B., Markwald, R. R. and Goodwin, R. L. (2009), Periostin promotes a fibroblastic lineage pathway in atrioventricular valve progenitor cells. Dev. Dyn., 238: 1052–1063. doi: 10.1002/dvdy.21933
- Issue published online: 21 APR 2009
- Article first published online: 30 MAR 2009
- Manuscript Accepted: 22 FEB 2009
- NIH-NHLBI. Grant Numbers: HL33756, 1KO2 HL086901, HL086856
- NIH-NCRR. Grant Number: COBRE P20RR016434-07
- National Science Foundation. Grant Number: FIBRE EF0526854
- Foundation Leducq. Grant Number: Mitral 07CVD04
- SC INBRE. Grant Number: 5MO1RR001070-28
- American Heart Association. Grant Number: 0765280U
Differentiation of prevalvular mesenchyme into valve fibroblasts is an integral step towards the development of functionally mature cardiac valves. Although clinically relevant, little is known regarding the molecular and cellular mechanisms by which this process proceeds. Genes that are regulated in a spatio-temporal pattern during valve remodeling are candidates for affecting this differentiation process. Based on its expression pattern, we have focused our studies on the role of the matricellular gene, periostin, in regulating the differentiation of cushion mesenchymal cells into valve fibroblasts. Herein, we demonstrate that periostin expression is coincident with and regulates type I collagen protein production, a major component of mature valve tissue. Adenoviral-mediated knock-down of periostin in atrioventricular mesenchyme resulted in a decrease in collagen I protein expression and aberrant induction of myocyte markers indicating an alteration in AV mesenchyme differentiation. In vitro analyses using a novel “cardiotube” assay further demonstrated that expression of periostin regulates lineage commitment of valve precursor cells. In these cells, expression of periostin and collagen I are regulated, in part, by TGFβ-3. We further demonstrate that TGFβ-3, through a periostin/collagen pathway, enhances the viscoelastic properties of AV cushion tissue surface tension and plays a crucial role in regulating valve remodeling. Thus, data presented here demonstrate that periostin, a TGFβ-3 responsive gene, functions as a crucial mediator of chick AV valve maturation via promoting mesenchymal-to-fibroblast differentiation while blocking differentiation of alternative cell types (myocytes). Developmental Dynamics 238:1052–1063, 2009. © 2009 Wiley-Liss, Inc.