Formaldehyde-based whole-mount in situ hybridization method for planarians
Article first published online: 19 MAR 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 4, page spcone, April 2009
How to Cite
Pearson, B. J., Eisenhoffer, G. T., Gurley, K. A., Rink, J. C., Miller, D. E. and Alvarado, A. S. (2009), Formaldehyde-based whole-mount in situ hybridization method for planarians. Dev. Dyn., 238: spcone. doi: 10.1002/dvdy.21937
- Issue published online: 19 MAR 2009
- Article first published online: 19 MAR 2009
- Manuscript Accepted: 30 NOV 2008
- NIH-NIGMS. Grant Numbers: RO-1 GM57260, F32GM082016
- Damon Runyon Foundation. Grant Number: 1888-05
- NIH. Grant Number: 5T32 HD07491
- European Molecular Biology Organization
- Cited By
Robust, high resolution formaldehyde-based in situ hybridization method for the interrogation of planarian regeneration. Schmidtea mediterranea specimen after head and tail regeneration labeled by combining immunofluorescence and double fluorescent in situ hybridization. Riboprobes for Smed-porcn-1 (gastrovascular system) and Smed-slit (midline cells) are shown in blue and green, respectively, while mouse anti-a-tubulin antibody (brain, ventral nerve chords, pharynx, flame cells) is shown in red/magenta. Images obtained by Sarah Elliott and Kyle Gurley, University of Utah. See Pearson et al., Developmental Dynamics 238:443-450. Developmental Dynamics, 2009. © 2009 Wiley-Liss, Inc.