Formaldehyde-based whole-mount in situ hybridization method for planarians

Authors

  • Bret J. Pearson,

    1. Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah
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  • George T. Eisenhoffer,

    1. Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah
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  • Kyle A. Gurley,

    1. Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah
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  • Jochen C. Rink,

    1. Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah
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  • Diane E. Miller,

    1. Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah
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  • Alejandro Sánchez Alvarado

    Corresponding author
    1. Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah
    • Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, 401 MREB, 20N 1900E, Salt Lake City, UT 84132
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Abstract

Robust, high resolution formaldehyde-based in situ hybridization method for the interrogation of planarian regeneration. Schmidtea mediterranea specimen after head and tail regeneration labeled by combining immunofluorescence and double fluorescent in situ hybridization. Riboprobes for Smed-porcn-1 (gastrovascular system) and Smed-slit (midline cells) are shown in blue and green, respectively, while mouse anti-a-tubulin antibody (brain, ventral nerve chords, pharynx, flame cells) is shown in red/magenta. Images obtained by Sarah Elliott and Kyle Gurley, University of Utah. See Pearson et al., Developmental Dynamics 238:443-450. Developmental Dynamics, 2009. © 2009 Wiley-Liss, Inc.

Ancillary