A novel role for the chemokine receptor Cxcr4 in kidney morphogenesis: An in vitro study
Article first published online: 21 APR 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 5, pages 1083–1091, May 2009
How to Cite
Ueland, J., Yuan, A., Marlier, A., Gallagher, A. R. and Karihaloo, A. (2009), A novel role for the chemokine receptor Cxcr4 in kidney morphogenesis: An in vitro study. Dev. Dyn., 238: 1083–1091. doi: 10.1002/dvdy.21943
- Issue published online: 21 APR 2009
- Article first published online: 21 APR 2009
- Manuscript Accepted: 5 MAR 2009
- Gottschalk Award from the American Society of Nephrology
- ureteric bud;
The CXCR4 chemokine receptor is involved in hematopoietic stem cell homing, neuronal development, and angiogenesis. We show a significant new role for this receptor in epithelial patterning and renal morphogenesis. This receptor is expressed in the ureteric bud (UB) and the metanephric mesenchyme (MM). Stimulation of Cxcr4 in renal tubular cells leads to activation of multiple signaling pathways and tubulogenesis and cell migration. Knocking down of this receptor in tubular cells leads to cyst formation. Inactivation of this receptor in embryonic kidney explants results in impaired UB branching and mesenchymal tubulogenesis. The data presented here point to its importance in the process of mesenchymal-to-epithelial transitioning (MET), a crucial developmental process in the embryonic kidney. A number of genes important for normal tubulogenesis and MET are decreased upon CXCR4 inactivation. Developmental Dynamics 238:1083–1091, 2009. © 2009 Wiley-Liss, Inc.