R.N.T. Lassiter and S.B. Reynolds contributed equally to this work.
FGF signaling is essential for ophthalmic trigeminal placode cell delamination and differentiation
Article first published online: 3 APR 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 5, pages 1073–1082, May 2009
How to Cite
Lassiter, R. N.T., Reynolds, S. B., Marin, K. D., Mayo, T. F. and Stark, M. R. (2009), FGF signaling is essential for ophthalmic trigeminal placode cell delamination and differentiation. Dev. Dyn., 238: 1073–1082. doi: 10.1002/dvdy.21949
- Issue published online: 21 APR 2009
- Article first published online: 3 APR 2009
- Manuscript Accepted: 4 MAR 2009
- NIH/NICHD. Grant Numbers: 5R03HD041470-02, 1R01HD046475-01
- BYU Undergraduate Mentored Research grant
- ophthalmic trigeminal placode;
- sensory neurogenesis;
The ophthalmic trigeminal (opV) placode gives rise exclusively to sensory neurons of the peripheral nervous system, providing an advantageous model for understanding neurogenesis. The signaling pathways governing opV placode development have only recently begun to be elucidated. Here, we investigate the fibroblast growth factor receptor-4 (FGFR4), an opV expressed gene, to examine if and how FGF signaling regulates opV placode development. After inhibiting FGFR4, Pax3+ opV placode cells failed to delaminate from the ectoderm and did not contribute to the opV ganglion. Blocking FGF signaling also led to a loss of the early and late neuronal differentiation markers Ngn2, Islet-1, NeuN, and Neurofilament. In addition, without FGF signaling, cells that stalled in the ectoderm lost their opV placode-specific identity by down-regulating Pax3. We conclude that FGF signaling, through FGFR4, is necessary for delamination and differentiation of opV placode cells. Developmental Dynamics 238:1073–1082, 2009. © 2009 Wiley-Liss, Inc.