R.N.T. Lassiter and S.B. Reynolds contributed equally to this work.
FGF signaling is essential for ophthalmic trigeminal placode cell delamination and differentiation
Version of Record online: 3 APR 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 5, pages 1073–1082, May 2009
How to Cite
Lassiter, R. N.T., Reynolds, S. B., Marin, K. D., Mayo, T. F. and Stark, M. R. (2009), FGF signaling is essential for ophthalmic trigeminal placode cell delamination and differentiation. Dev. Dyn., 238: 1073–1082. doi: 10.1002/dvdy.21949
- Issue online: 21 APR 2009
- Version of Record online: 3 APR 2009
- Manuscript Accepted: 4 MAR 2009
- NIH/NICHD. Grant Numbers: 5R03HD041470-02, 1R01HD046475-01
- BYU Undergraduate Mentored Research grant
Additional Supporting Information may be found in the online version of this article.
|DVDY_21949_sm_SupplFigS1.tif||967K||Supp. Fig. 1. Inhibition of fibroblast growth factor (FGF) signaling prevents targeted cells from delaminating and contributing to the ophthalmic trigeminal (opV) ganglion. A–C: Transverse section through the opV ganglion region of a ∼31 somite stage (ss) embryo collected 36 hr after electroporation at the 7–9 ss with the truncated FGF receptor-4 (tFGFR4) vector (green; A), immunostained for Pax3 (red; B) and Islet1 (blue; C). D: Merged image of tFGFR4 and Pax3; GFP+ tFGFR4-targeted cells remain in the ectoderm, do not express Pax3, and do not contribute to the ganglion. E: Merged image of tFGFR4 and Islet1; targeted tFGFR4 cells remain in the ectoderm and do not express Islet1.|
|DVDY_21949_sm_SupplFigS2.tif||2266K||Supp. Fig. 2. Fibroblast growth factor-8 (FGF8) misexpression does not increase delamination of placode cells or differentiation. A–C,F–H: Transverse section through the opV ganglion region of ∼25 somite stage (ss) or ∼31 ss embryos electroporated at the 7–9 ss with the FGF8 vector (green; A,F) and collected at 24 hr (A–E) or 36 hr (F–J), immunostained for Pax3 (red; B,G) and Islet1 (blue; C,H). D: Merged image of FGF8 and Pax3; green fluorescent protein–positive (GFP+) FGF8-targeted cells at 24 hr after electroporation do not show an increase in Pax3+ cells or delamination compared with controls. E: Merged image of FGF8 and Islet1. Targeted cells do not show an increase in Islet1 expression. I: Merged image of FGF8 and Pax3; GFP+ FGF8-targeted cells 36 hr after electroporation do not show evidence of ectopic ganglia or an increase in the number of Pax3 cells delaminating and contributing to the opV ganglion. J: Merged image of FGF8 and Islet1 at 36 hr after electroporation do not show an increase in Islet1 expression.|
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