Embryogenesis and laboratory maintenance of the foam-nesting túngara frogs, genus Engystomops (= Physalaemus)

Authors

  • Andrés Romero-Carvajal,

    1. Laboratorio de Biología del Desarrollo, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
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  • Natalia Sáenz-Ponce,

    1. Laboratorio de Biología del Desarrollo, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
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  • Michael Venegas-Ferrín,

    1. Laboratorio de Biología del Desarrollo, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
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  • Diego Almeida-Reinoso,

    1. Museo de Zoología, Sección de Herpetología, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
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  • Chanjae Lee,

    1. Section of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology University of Texas, Austin, Texas
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  • Jennifer Bond,

    1. Section of Integrative Biology, University of Texas, Austin, Texas
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  • Michael J. Ryan,

    1. Section of Integrative Biology, University of Texas, Austin, Texas
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  • John B. Wallingford,

    1. Section of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology University of Texas, Austin, Texas
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  • Eugenia M. del Pino

    Corresponding author
    1. Laboratorio de Biología del Desarrollo, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
    • Pontificia Universidad Católica del Ecuador, Escuela de Ciencias Biológicas, Apartado 17-01-2184, Avenida 12 de Octubre 1076 y Roca, Quito, Ecuador
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Abstract

The vast majority of embryological research on amphibians focuses on just a single genus of frogs, Xenopus. To attain a more comprehensive understanding of amphibian development, experimentation on non-model frogs will be essential. Here, we report on the early development, rearing, and embryological analysis of túngara frogs (genus Engystomops, also called Physalaemus). The frogs Engystomops pustulosus, Engystomops coloradorum, and Engystomops randi construct floating foam-nests with small eggs. We define a table of 23 stages for the developmental period in the foam-nest. Embryos were immunostained against Lim1, neural, and somite-specific proteins and the expression pattern of RetinoBlastoma Binding Protein 6 (RBBP6) was analyzed by in situ hybridization. Due to their brief life-cycle, frogs belonging to the genus Engystomops are attractive for comparative and genetic studies of development. Developmental Dynamics 238:1444–1454, 2009. © 2009 Wiley-Liss, Inc.

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