Morphometric analysis of testis cord formation in Sox9-EGFP mice
Version of Record online: 21 APR 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 5, pages 1100–1110, May 2009
How to Cite
Nel-Themaat, L., Vadakkan, T. J., Wang, Y., Dickinson, M. E., Akiyama, H. and Behringer, R. R. (2009), Morphometric analysis of testis cord formation in Sox9-EGFP mice. Dev. Dyn., 238: 1100–1110. doi: 10.1002/dvdy.21954
- Issue online: 21 APR 2009
- Version of Record online: 21 APR 2009
- Manuscript Accepted: 12 MAR 2009
- National Institutes of Health. Grant Numbers: HD30284, HD07324, HL077187, EB005173, EB007076
- Ben F. Love Endowed Chair
- Sertoli cells;
- sex determination;
- green fluorescent protein;
Sox9-EGFP knockin mice were generated to label Sertoli cells and visualize testis cord formation during development. Confocal microscopy and morphometric analysis of developing cords were performed. Serial histological sections were used for three-dimensional cord reconstruction. Initially, gonad length decreased from embryonic day (E) 11.5 to E13.5, but increased thereafter, while gonad width doubled every 12 hours from E11.5 through E14.5. At E12.5, the average number of cords was 12.5, whereas this decreased to 10.4 at E13.5 and E14.5. Cord number at a given time point varied between gonads and influenced dimensions. The initial cords that formed were complex and branches were common. Time-lapse imaging revealed an intricate behavior of the Sertoli–germ cell mass and cellular exchange between connected neighboring cords. These results suggest that cord formation is a highly dynamic process that subsequently becomes refined to establish the final number of seminiferous tubule precursors. Developmental Dynamics 238:1100–1110, 2009. © 2009 Wiley-Liss, Inc.