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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
DVDY_21954_sm_SupMovieS1A.avi11679KSupp. Movie S1A. Sox9-EGFP testis at embryonic day (E) 11.5 cultured and imaged for 15 hr at 30-min intervals. The mesonephros is to the right and the distal edge to the left of the green Sertoli–germ cell mass (SGCM). Initially, the distal edge appears smooth, but become serrated after approximately 6 hr when single Sertoli cell extensions become visible, followed by more defined small protrusions of the cell mass into the surrounding tissue. The gonad also becomes progressively thicker while enhanced green fluorescent protein (EGFP) intensity increases.
DVDY_21954_sm_SupMovieS1B.avi22263KSupp. Movie S1B. Sox9-EGFP testis at embryonic day (E) 12.0 cultured and imaged for 18 hr at 20-min intervals. The mesonephros is at the bottom and the distal edge at the top of the green Sertoli–germ cell mass (SGCM). Small protrusions on the distal edge become more defined and elongate while the length of the gonad decreases.
DVDY_21954_sm_SupMovieS1C.avi33868KSupp. Movie S1C. Sox9-EGFP testis at embryonic day (E) 12.5 cultured and imaged for 43.5 hr at 30-min intervals. The mesonephros is at the top and the distal edge at the bottom of the green Sertoli–germ cell mass (SGCM). Well-defined, small protrusions enlarge and fuse to form large cord-like structures, while the cord number decreases over time. The gonad length also decreases, while the width increases as cords elongate. Nonfluorescing areas form in the SGCM, that seemingly merge with one another to contribute to the interstitial spaces between the cords.
DVDY_21954_sm_SupMovieS2A.avi32098KSupp. Movie S2A. Animation of a three-dimensional reconstruction model showing the morphology of single testis cord A (wire frame format using Imaris 5.0.3) (Fig. 10D).
DVDY_21954_sm_SupMovieS2B.avi23871KSupp. Movie S2B. Animation of a three-dimensional reconstruction model showing the morphology of single testis cord B (isosurface through equal intensity points using Imaris 5.0.3) (Fig. 10E).

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